Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-5-1
pubmed:databankReference
pubmed:abstractText
Five domains of antigenic importance were previously mapped on the nucleocapsid protein (N) of the porcine reproductive and respiratory syndrome virus (PRRSV), and a domain comprised of the 11 C-terminal-most amino acids (residues 112 to 123) was shown to be essential for binding of N-specific conformation-dependent monoclonal antibodies (MAbs). In the present study, the importance of individual residues within this C-terminal domain for antigenicity was investigated using eight different mutant constructs of N expressed in HeLa cells. Single amino acid substitutions were introduced into the C-terminal domain of the N protein, and the significance of individual amino acids for MAb reactivity was determined by immunoprecipitation. None of the MAbs tested recognized the mutant with a leucine-to-proline substitution at residue 114 (L114P), while V112P, R113P, R113D, I115P, and R116P reduced MAb binding significantly. Conversely, substitution of amino acids at positions 118 (T118S) and 121 (P121A) had little effect on MAb binding. Secondary-structure predictions indicate that amino acids 111 to 117 form a beta-strand. In view of the fact that replacement of beta-strand-forming amino acids with proline elicited the greatest effect on MAb binding, it appears that secondary structure in the C terminus of the N protein is an important determinant of conformational epitope formation. While the crystal structure of the PRRSV N protein remains to be determined, results from these studies broaden our understanding of the secondary structures that make up the PRRSV N protein and shed some light on how they may relate to function.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-10073689, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-10226618, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-10400725, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-10518575, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-10726638, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-10856758, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-11003472, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-11205119, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-2421482, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-592361, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-7508455, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-7578443, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-7782765, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-7794115, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-8438574, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-8517032, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-8574824, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-8645111, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9186940, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9220627, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9292014, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9349308, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9545131, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9660067, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9801333, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9847330, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9875321, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329465-9927721
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
598-603
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Antigenic importance of the carboxy-terminal beta-strand of the porcine reproductive and respiratory syndrome virus nucleocapsid protein.
pubmed:affiliation
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't