Linked Life Data

11328596

Source:http://linkedlifedata.com/resource/pubmed/id/11328596

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-4-30
pubmed:abstractText
CD63 is located on the basophilic granule membranes in resting basophils, mast cells, and platelets, and is also located on the plasma membranes of the cells. We constructed a CD63-GFP (green fluorescent protein) plasmid and introduced it into rat basophilic leukemia (RBL-2H3) cells to observe the movements of CD63 on degranulation. The movements of CD63-GFP were studied in living RBL cells by confocal laser scanning microscopy (CLSM). CD63-GFP, in which GFP was conjugated to the C-terminus of CD63, was located on both the granule membranes and the plasma membranes of RBL cells. The diameter of the fluorescent granules in the cytoplasm varied from 0.5 to 1.5 microm. Before antigen stimulation most granules with CD63-GFP hardly moved in RBL cells. However, after antigen stimulation the plasma membranes ruffled violently and the granules moved dramatically. They reached the plasma membranes in a few minutes and fused with them instantaneously. Analysis of the movement of each granule provided a new insight into the elementary process of degranulation. The velocity of the granule movement toward the plasma membranes on antigen stimulation was calculated to be 0.1+/-0.02 microm/s. This shows that the granules are able to reach the plasma membranes in 2 or 3 min if the diameter of the cells is 20 microm.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
739-44
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11328596-Animals, pubmed-meshheading:11328596-Antigens, CD, pubmed-meshheading:11328596-Antigens, CD63, pubmed-meshheading:11328596-Cell Degranulation, pubmed-meshheading:11328596-Cell Membrane, pubmed-meshheading:11328596-Cytoplasmic Granules, pubmed-meshheading:11328596-Cytoplasmic Streaming, pubmed-meshheading:11328596-Green Fluorescent Proteins, pubmed-meshheading:11328596-Immunization, pubmed-meshheading:11328596-Leukemia, Basophilic, Acute, pubmed-meshheading:11328596-Luminescent Proteins, pubmed-meshheading:11328596-Microscopy, Confocal, pubmed-meshheading:11328596-Plasmids, pubmed-meshheading:11328596-Platelet Membrane Glycoproteins, pubmed-meshheading:11328596-Rats, pubmed-meshheading:11328596-Tumor Cells, Cultured, pubmed-meshheading:11328596-beta-N-Acetylhexosaminidases
pubmed:year
2001
pubmed:articleTitle
Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells.
pubmed:affiliation
Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan. mamoru@phar.nagoya-cu.ac.jp
pubmed:publicationType
Journal Article