Linked Life Data

11327767

Source:http://linkedlifedata.com/resource/pubmed/id/11327767

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-4-30
pubmed:abstractText
Sensory rhodopsins, phototaxis receptors in Haloarchaea, were purified and reconstituted into halobacterial lipids to form photoactive two-dimensional crystals. Images of vitreous ice-embedded, flattened, tubular crystals of sensory rhodopsin II (SRII) of Natronobacterium pharaonis were recorded using a field emission gun electron cryo-microscope. Fourier components for the SRII structure were determined either from the separated image transforms from single layers that formed each side of flattened tubes, or by a deconvolution procedure when two layers were stacked in register so that they generated a single crystal lattice by superposition. Most micrographs showed significant diffraction to 6.9 A after computer processing, and the results provide the first intermediate- resolution information obtained for an archaeal sensory rhodopsin. The projection structure of SRII indicates that the helix positions match the seven-helix arrangement of the archaeal transport rhodopsins rather than that of the eukaryotic visual pigments. The structural similarity of SRII to the transport rhodopsins supports models in which the transport and signalling mechanisms of archaeal rhodopsins derive from the same retinal-driven changes in protein conformation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2836
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
308
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-93
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11327767-Animals, pubmed-meshheading:11327767-Archaeal Proteins, pubmed-meshheading:11327767-Bacteriorhodopsins, pubmed-meshheading:11327767-Carotenoids, pubmed-meshheading:11327767-Cattle, pubmed-meshheading:11327767-Cryoelectron Microscopy, pubmed-meshheading:11327767-Crystallization, pubmed-meshheading:11327767-Fourier Analysis, pubmed-meshheading:11327767-Halobacterium salinarum, pubmed-meshheading:11327767-Halorhodopsins, pubmed-meshheading:11327767-Image Processing, Computer-Assisted, pubmed-meshheading:11327767-Models, Molecular, pubmed-meshheading:11327767-Natronobacterium, pubmed-meshheading:11327767-Protein Conformation, pubmed-meshheading:11327767-Recombinant Fusion Proteins, pubmed-meshheading:11327767-Rhodopsin, pubmed-meshheading:11327767-Sensory Rhodopsins
pubmed:year
2001
pubmed:articleTitle
Electron crystallographic analysis of two-dimensional crystals of sensory rhodopsin II: a 6.9 A projection structure.
pubmed:affiliation
MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't