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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2001-4-26
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pubmed:abstractText |
We have used a yeast two-hybrid approach to uncover protein interactions involving the D2-like subfamily of dopamine receptors. Using the third intracellular loop of the D2S and D3 dopamine receptors as bait to screen a human brain cDNA library, we identified filamin A (FLN-A) as a protein that interacts with both the D2 and D3 subtypes. The interaction with FLN-A was specific for the D2 and D3 receptors and was independently confirmed in pull-down and coimmunoprecipitation experiments. Deletion mapping localized the dopamine receptor-FLN-A interaction to the N-terminal segment of the D2 and D3 dopamine receptors and to repeat 19 of FLN-A. In cultures of dissociated rat striatum, FLN-A and D2 receptors colocalized throughout neuronal somata and processes as well as in astrocytes. Expression of D2 dopamine receptors in FLN-A-deficient M2 melanoma cells resulted in predominant intracellular localization of the D2 receptors, whereas in FLN-A-reconstituted cells, the D2 receptor was predominantly localized at the plasma membrane. These results suggest that FLN-A may be required for proper cell surface expression of the D2 dopamine receptors. Association of D2 and D3 dopamine receptors with FLN-A provides a mechanism whereby specific dopamine receptor subtypes may be functionally linked to downstream signaling components via the actin cytoskeleton.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10051605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10373412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10377350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10617615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10659839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10692483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10753124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10890919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-10999936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-11163774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-1332033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-1833070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-2042108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-2391361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-2594196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-2856162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-4063818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-6879197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-7534799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-7569905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-7904756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-8384581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-8494342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9006895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9006936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9009191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9367441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9437013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9636219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9759378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9804783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9853904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9883725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9891976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11320256-9892354
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Contractile Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DRD3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Drd3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D3,
http://linkedlifedata.com/resource/pubmed/chemical/filamins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5258-63
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11320256-Actins,
pubmed-meshheading:11320256-Animals,
pubmed-meshheading:11320256-Binding Sites,
pubmed-meshheading:11320256-Cells, Cultured,
pubmed-meshheading:11320256-Contractile Proteins,
pubmed-meshheading:11320256-Cytoskeleton,
pubmed-meshheading:11320256-Fluorescent Antibody Technique,
pubmed-meshheading:11320256-Humans,
pubmed-meshheading:11320256-Melanoma,
pubmed-meshheading:11320256-Microfilament Proteins,
pubmed-meshheading:11320256-Models, Biological,
pubmed-meshheading:11320256-Neostriatum,
pubmed-meshheading:11320256-Precipitin Tests,
pubmed-meshheading:11320256-Protein Binding,
pubmed-meshheading:11320256-Protein Structure, Tertiary,
pubmed-meshheading:11320256-Rats,
pubmed-meshheading:11320256-Receptors, Dopamine D2,
pubmed-meshheading:11320256-Receptors, Dopamine D3,
pubmed-meshheading:11320256-Sequence Deletion,
pubmed-meshheading:11320256-Substrate Specificity,
pubmed-meshheading:11320256-Tumor Cells, Cultured,
pubmed-meshheading:11320256-Two-Hybrid System Techniques
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pubmed:year |
2001
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pubmed:articleTitle |
Dopamine D2 and D3 receptors are linked to the actin cytoskeleton via interaction with filamin A.
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pubmed:affiliation |
Neuroscience Graduate Program, Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA.
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pubmed:publicationType |
Journal Article
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