Linked Life Data

11319826

Source:http://linkedlifedata.com/resource/pubmed/id/11319826

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-4-26
pubmed:abstractText
Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature for up to 1 month in the ethylene glycol-sodium acetate solvent system without the requirement for a nitrogen atmosphere. The reaction was very accurate and precise, with intra- and interassay variations of less than 3% when 5 microg of protein was assayed. All proteins that were investigated contributed the same color intensity per microgram protein as bovine serum albumin. This assay was several times more sensitive than the Coomassie reaction and linear over a greater range of protein concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
292
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-9
pubmed:dateRevised
2009-11-3
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
A ninhydrin-based assay to quantitate the total protein content of tissue samples.
pubmed:affiliation
University of Texas Health Center at Tyler, Tyler, Texas 75708, USA. starcher@uthct.edu
pubmed:publicationType
Journal Article, Comparative Study