Linked Life Data

11319166

Source:http://linkedlifedata.com/resource/pubmed/id/11319166

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-4-25
pubmed:databankReference
pubmed:abstractText
Chicken vasoactive intestinal polypeptide receptor (VIPR) cDNA was cloned by the reverse transcription-polymerase chain reaction method using primers designed on the basis of other species of VIPR cDNA. The cDNA obtained was sequenced by the dideoxy-mediated chain-termination method. Of the 2227 nucleotides that were sequenced, 84, 855, and 1338 bases represent the 5'-untranslated region (UTR), the 3'-UTR, and the open reading frame that predicts a peptide of 446 amino acids. The cDNA of the chicken VIPR shows 65% and 60% homologies to human cDNA of VIP1 and VIP2 receptors, respectively. The clone had the expected similarity to highly conserved features of the other G protein-coupled receptors (GPCRs) such as six cysteine residues that are functionally important in the VIPR subfamily. In addition, the seven potential membrane-spanning domains characteristic of the family B group III GPCR superfamily and highly conserved motif within the third cellular loop between transmembrane regions 5 and 6. Northern blot hybridization analysis in this study indicated mRNA expression of VIPRs in the various tissues of the chicken. Strong signal was detected in the brain and anterior pituitary gland. High levels of VIPR mRNA in the brain was consistent with VIP-binding experiments and with the function of VIP in the brain as a neuroendocrine factor or neurotransmitter. Expression of VIPR was detected in the anterior pituitary gland of chick embryos. The expression of VIPR mRNA in the chick anterior pituitary gland may indicate a regulatory function of VIP on prolactin (PRL) production or PRL cell proliferation during embryogenesis. Chicken VIPR shows high homology with mammalian type I VIPR but, in some part, possesses similarity of amino acid sequence. Expression of VIPR in various tissues supports diverse functions for VIP in the chicken.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1575-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11319166-3' Untranslated Regions, pubmed-meshheading:11319166-5' Untranslated Regions, pubmed-meshheading:11319166-Amino Acid Sequence, pubmed-meshheading:11319166-Animals, pubmed-meshheading:11319166-Base Sequence, pubmed-meshheading:11319166-Blotting, Northern, pubmed-meshheading:11319166-Brain Chemistry, pubmed-meshheading:11319166-Chickens, pubmed-meshheading:11319166-Chromosome Mapping, pubmed-meshheading:11319166-Cloning, Molecular, pubmed-meshheading:11319166-DNA, Complementary, pubmed-meshheading:11319166-Female, pubmed-meshheading:11319166-Humans, pubmed-meshheading:11319166-In Situ Hybridization, Fluorescence, pubmed-meshheading:11319166-Molecular Sequence Data, pubmed-meshheading:11319166-Nucleic Acid Hybridization, pubmed-meshheading:11319166-Open Reading Frames, pubmed-meshheading:11319166-Organ Specificity, pubmed-meshheading:11319166-Phylogeny, pubmed-meshheading:11319166-Pituitary Gland, Anterior, pubmed-meshheading:11319166-Receptors, Vasoactive Intestinal Peptide, pubmed-meshheading:11319166-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2001
pubmed:articleTitle
Molecular cloning of chicken vasoactive intestinal polypeptide receptor complementary DNA, tissue distribution and chromosomal localization.
pubmed:affiliation
Laboratory of Animal Physiology, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan 464-860.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't