Source:http://linkedlifedata.com/resource/pubmed/id/11318414
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2001-4-24
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pubmed:abstractText |
Cytosine arabinoside (Ara-C) is widely used to induce remission in adult granulocytic leukemia. High doses can be infused in refractory leukemia or in relapse. After injection, Ara-C is quickly metabolized to uracil arabinoside (Ara-U), the main inactive metabolite. We here described a micellar electrokinetic capillary chromatography (MECC) method to simultaneously determine Ara-C/Ara-U in human serum using 6-O-methylguanine as an internal standard. The assay was linear from 6.25 to 200 microg/ml with a quantification limit between 3 and 6 microg/ml. The analytical precision was satisfactory between 2 and 4.3% (within-run) and 3.7 and 7.3% (between-runs). This assay was applied to the analysis of serum from acute granulocytic leukemia patient treated by high doses cytarabine (3 g/m2 body surface).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1387-2273
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
754
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
185-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11318414-Antimetabolites, Antineoplastic,
pubmed-meshheading:11318414-Arabinofuranosyluracil,
pubmed-meshheading:11318414-Chromatography, Micellar Electrokinetic Capillary,
pubmed-meshheading:11318414-Cytarabine,
pubmed-meshheading:11318414-Humans,
pubmed-meshheading:11318414-Leukemia, Myeloid,
pubmed-meshheading:11318414-Osmolar Concentration,
pubmed-meshheading:11318414-Reproducibility of Results,
pubmed-meshheading:11318414-Sensitivity and Specificity,
pubmed-meshheading:11318414-Temperature
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pubmed:year |
2001
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pubmed:articleTitle |
Micellar electrokinetic capillary chromatography quantification of cytosine arabinoside and its metabolite, uracil arabinoside, in human serum.
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pubmed:affiliation |
Laboratoire de Biochmie A, H pital Saint Louis, Paris, France.
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pubmed:publicationType |
Journal Article,
Comparative Study
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