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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-4-24
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pubmed:abstractText |
Ferritin was purified from iron-fed Galleria mellonella hemolymph by ultra centrifugation and FPLC (Superose 6). SDS-PAGE revealed three bands of 26, 30, and 32 kDa. The ferritin 26 kDa subunit cDNA was obtained from RT-PCR using primer designed from N-terminal sequence analysis. 5'-RACE was used to obtain the complete protein coding sequence. The sequence encodes a 211 amino acid polypeptide including a 20 amino acid leader peptide. An IRE (iron-responsive element) sequence with a predicted stem-loop structure was present in the 5'-UTR of ferritin mRNA. Sequence alignment has a sequence identity with Calpodes ethlius (S)(74%), Drosophila melanogaster (50%), and Aedes aegypti (39%). Northern blot analysis indicated that there were 1.5- and 1.75-fold increases in the expression of ferritin mRNA after iron-fed fat body and midgut, respectively. Also, we confirmed that the ferritin mRNA is not expressed in adult ovary and testis. Arch.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0739-4462
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8-17
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11317331-Amino Acid Sequence,
pubmed-meshheading:11317331-Animals,
pubmed-meshheading:11317331-Base Sequence,
pubmed-meshheading:11317331-Cloning, Molecular,
pubmed-meshheading:11317331-DNA, Complementary,
pubmed-meshheading:11317331-Female,
pubmed-meshheading:11317331-Ferritins,
pubmed-meshheading:11317331-Gene Expression,
pubmed-meshheading:11317331-Humans,
pubmed-meshheading:11317331-Insect Proteins,
pubmed-meshheading:11317331-Iron,
pubmed-meshheading:11317331-Male,
pubmed-meshheading:11317331-Molecular Sequence Data,
pubmed-meshheading:11317331-Moths,
pubmed-meshheading:11317331-RNA, Messenger,
pubmed-meshheading:11317331-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:11317331-Sequence Homology, Amino Acid,
pubmed-meshheading:11317331-Tissue Distribution
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pubmed:year |
2001
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pubmed:articleTitle |
Cloning and expression of a ferritin subunit for Galleria mellonella.
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pubmed:affiliation |
Department of Biology, Korea University, Seoul, Korea.
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pubmed:publicationType |
Journal Article
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