Linked Life Data

11313889

Source:http://linkedlifedata.com/resource/pubmed/id/11313889

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-4-23
pubmed:databankReference
pubmed:abstractText
In a search for molecular markers of progression in prostate cancer by means of differential display, we have identified a new gene, which we have designated PTOV1. Semiquantitative RT-PCR has established that nine out of 11 tumors overexpress PTOV1 at levels significantly higher than benign prostatic hyperplasia or normal prostate tissue. The human PTOV1 protein consists almost entirely of two repeated blocks of homology of 151 and 147 amino acids, joined by a short linker peptide, and is encoded by a 12-exon gene localized in chromosome 19q13.3. A Drosophila melanogaster PTOV1 homolog also contains two tandemly arranged PTOV blocks. A second gene, PTOV2, was identified in humans and Drosophila, coding for proteins with a single PTOV homology block and unrelated amino- and carboxyl-terminal extensions. A 1.8-Kb PTOV1 transcript was detected abundantly in normal human brain, heart, skeletal muscle, kidney and liver, and at low levels in normal prostate. Immunocytochemical analysis and expression of chimeric GFP-PTOV1 proteins in cultured cells showed a predominantly perinuclear localization of PTOV1. In normal prostate tissue and in prostate adenomas, PTOV1 was undetectable or expressed at low levels, whereas nine out of 11 prostate adenocarcinomas showed a strong immunoreactivity, with a focal distribution in areas of carcinoma and prostatic intraepithelial neoplasia. Therefore, PTOV1 is a previously unknown gene, overexpressed in early and late stages of prostate cancer. The PTOV homology block represents a new class of conserved sequence blocks present in human, rodent and fly proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1455-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11313889-Adenocarcinoma, pubmed-meshheading:11313889-Amino Acid Sequence, pubmed-meshheading:11313889-Base Sequence, pubmed-meshheading:11313889-Cell Compartmentation, pubmed-meshheading:11313889-Chromosome Mapping, pubmed-meshheading:11313889-Chromosomes, Human, Pair 19, pubmed-meshheading:11313889-Databases, Factual, pubmed-meshheading:11313889-Drosophila Proteins, pubmed-meshheading:11313889-Humans, pubmed-meshheading:11313889-In Situ Hybridization, Fluorescence, pubmed-meshheading:11313889-Male, pubmed-meshheading:11313889-Molecular Sequence Data, pubmed-meshheading:11313889-Neoplasm Proteins, pubmed-meshheading:11313889-Prostatic Hyperplasia, pubmed-meshheading:11313889-Prostatic Neoplasms, pubmed-meshheading:11313889-Proteins, pubmed-meshheading:11313889-RNA, Messenger, pubmed-meshheading:11313889-RNA, Neoplasm, pubmed-meshheading:11313889-Recombinant Fusion Proteins, pubmed-meshheading:11313889-Sequence Homology, Amino Acid, pubmed-meshheading:11313889-Tissue Distribution, pubmed-meshheading:11313889-Tumor Markers, Biological
pubmed:year
2001
pubmed:articleTitle
PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks.
pubmed:affiliation
Unitat de Recerca Biomèdica, Hospital Materno-Infantil, Hospitals Vall d'Hebrón, Barcelona, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't