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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2001-4-23
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pubmed:abstractText |
The glycosylphosphatidylinositol-anchored CD24 protein is a B cell differentiation Ag that is expressed on mature resting B cells but disappears upon Ag stimulation. We used Burkitt's lymphoma (BL) cells, which are thought to be related to germinal center B cells, to examine the biological effect of Ab-mediated CD24 cross-linking on human B cells and observed 1) induction of apoptosis in BL cells mediated by cross-linking of CD24; and 2) synergism between the cross-linking of CD24 and that of the B cell receptor for Ag in the effect on apoptosis induction. We also observed activation of mitogen-activated protein kinases following CD24 cross-linking, suggesting that CD24 mediates the intracellular signaling that leads to apoptosis in BL cells. Although CD24 has no cytoplasmic portion to transduce signals intracellularly, analysis of biochemically separated glycolipid-enriched membrane (GEM) fractions indicated enhanced association of CD24 and Lyn protein tyrosine kinase in GEM as well as increased Lyn kinase activity after CD24 cross-linking, suggesting that CD24 mediates intracellular signaling via a GEM-dependent mechanism. Specific microscopic cocapping of CD24 and Lyn, but not of other kinases, following CD24 cross-linking supported this idea. We further observed that apoptosis induction by cross-linking is a common feature shared by GEM-associated molecules expressed on BL cells, including GPI-anchored proteins and glycosphingolipids. CD24-mediated apoptosis in BL cells may provide a model for the cell death mechanism initiated by GEM-associated molecules, which is closely related to B cell receptor for Ag-mediated apoptosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD24,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Biklk protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/CD24 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/lyn protein-tyrosine kinase,
http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
166
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5567-77
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:11313396-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:11313396-Antibodies, Monoclonal,
pubmed-meshheading:11313396-Antigens, CD,
pubmed-meshheading:11313396-Antigens, CD24,
pubmed-meshheading:11313396-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:11313396-Apoptosis,
pubmed-meshheading:11313396-B-Lymphocytes,
pubmed-meshheading:11313396-Biological Transport, Active,
pubmed-meshheading:11313396-Burkitt Lymphoma,
pubmed-meshheading:11313396-Carrier Proteins,
pubmed-meshheading:11313396-Cell Fractionation,
pubmed-meshheading:11313396-Cell Membrane,
pubmed-meshheading:11313396-Centrifugation, Density Gradient,
pubmed-meshheading:11313396-Cholera Toxin,
pubmed-meshheading:11313396-Glycosylphosphatidylinositols,
pubmed-meshheading:11313396-Humans,
pubmed-meshheading:11313396-Immune Sera,
pubmed-meshheading:11313396-Intracellular Fluid,
pubmed-meshheading:11313396-Membrane Glycoproteins,
pubmed-meshheading:11313396-Membrane Microdomains,
pubmed-meshheading:11313396-Mitochondrial Proteins,
pubmed-meshheading:11313396-Receptors, Antigen, B-Cell,
pubmed-meshheading:11313396-Signal Transduction,
pubmed-meshheading:11313396-Tumor Cells, Cultured,
pubmed-meshheading:11313396-src-Family Kinases
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pubmed:year |
2001
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pubmed:articleTitle |
CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system.
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pubmed:affiliation |
Department of Pathology, National Children's Medical Research Center, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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