Source:http://linkedlifedata.com/resource/pubmed/id/11313370
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2001-4-23
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| pubmed:abstractText |
The role of viral structural proteins in the initiation of adaptive immune responses is poorly understood. To address this issue, we focused on the effect of noninfectious papillomavirus-like particles (VLPs) on dendritic cell (DC) activation. We found that murine bone marrow-derived dendritic cells (BMDCs) effectively bound and rapidly internalized bovine papillomavirus VLPS: Exposure to fully assembled VLPs of bovine papillomavirus, human papillomavirus (HPV)16 or HPV18, but not to predominately disordered HPV16 capsomers, induced acute phenotypic maturation of BMDCS: Structurally similar polyomavirus VLPs bound to the DC surface and were internalized, but failed to induce maturation. DCs that had incorporated HPV16 VLPs produced proinflammatory cytokines IL-6 and TNF-alpha; however, the release of these cytokines was delayed relative to LPS activation. Production of IL-12p70 by VLP-exposed DCs required the addition of syngeneic T cells or rIFN-gamma. Finally, BMDCs pulsed with HPV16 VLPs induced Th1-dominated primary T cell responses in vitro. Our data provide evidence that DCs respond to intact papillomavirus capsids and that they play a central role in VLP-induced immunity. These results offer a mechanistic explanation for the striking ability of papillomavirus VLP-based vaccines to induce potent T and B cell responses even in the absence of adjuvant.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/L1 protein, Bovine papillomavirus,
http://linkedlifedata.com/resource/pubmed/chemical/L1 protein, Human papillomavirus...,
http://linkedlifedata.com/resource/pubmed/chemical/L2 protein, Bovine papillomavirus,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral
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| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
166
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5346-55
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11313370-Animals,
pubmed-meshheading:11313370-BK Virus,
pubmed-meshheading:11313370-Bovine papillomavirus 1,
pubmed-meshheading:11313370-Capsid,
pubmed-meshheading:11313370-Capsid Proteins,
pubmed-meshheading:11313370-Cattle,
pubmed-meshheading:11313370-Cell Differentiation,
pubmed-meshheading:11313370-Cells, Cultured,
pubmed-meshheading:11313370-Cytokines,
pubmed-meshheading:11313370-Dendritic Cells,
pubmed-meshheading:11313370-Humans,
pubmed-meshheading:11313370-Immunophenotyping,
pubmed-meshheading:11313370-Inflammation Mediators,
pubmed-meshheading:11313370-Interphase,
pubmed-meshheading:11313370-JC Virus,
pubmed-meshheading:11313370-Lymphocyte Activation,
pubmed-meshheading:11313370-Mice,
pubmed-meshheading:11313370-Mice, Inbred C57BL,
pubmed-meshheading:11313370-Oncogene Proteins, Viral,
pubmed-meshheading:11313370-Papillomaviridae,
pubmed-meshheading:11313370-Protein Binding,
pubmed-meshheading:11313370-Th1 Cells,
pubmed-meshheading:11313370-Virion,
pubmed-meshheading:11313370-Virus Assembly
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| pubmed:year |
2001
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| pubmed:articleTitle |
Papillomavirus-like particles induce acute activation of dendritic cells.
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| pubmed:affiliation |
Laboratory of Cellular Oncology, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|