| pubmed-article:11312696 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11312696 | lifeskim:mentions | umls-concept:C0032846 | lld:lifeskim |
| pubmed-article:11312696 | lifeskim:mentions | umls-concept:C0004597 | lld:lifeskim |
| pubmed-article:11312696 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:11312696 | lifeskim:mentions | umls-concept:C0009276 | lld:lifeskim |
| pubmed-article:11312696 | lifeskim:mentions | umls-concept:C0009399 | lld:lifeskim |
| pubmed-article:11312696 | lifeskim:mentions | umls-concept:C1407029 | lld:lifeskim |
| pubmed-article:11312696 | lifeskim:mentions | umls-concept:C0998633 | lld:lifeskim |
| pubmed-article:11312696 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11312696 | pubmed:dateCreated | 2001-4-23 | lld:pubmed |
| pubmed-article:11312696 | pubmed:abstractText | We expressed the wild-type cry3Aa3 and cry3Ca1 Bacillus thuringiensis genes, which code for insecticidal proteins, in an Escherichia coli expression system. Highly purified preparations of the soluble delta-endotoxins were used to perform comparative bioassays with third-instar larvae of the Colorado potato beetle (CPB). Acute mortality data showed that Cry3Ca1 (LD(50) = 320.1 ng) was 2-fold more toxic than Cry3Aa3 (LD(50) = 672.9 ng). We also compared the chronic effects of sublethal doses of these toxins by measuring the consumption of untreated foliage and monitoring survival and development for 6 days after intoxication. No significant additional mortality was recorded, but we found that surviving larvae fed Cry3Ca1 consumed foliage at a slower rate than the larvae fed Cry3Aa3, suggesting more damage to their digestive epithelium. This study, the first assessment of the toxicity of cry3Ca1 in third-instar CPB, suggests cry3Ca1 will prove useful for the control of this important insect pest. | lld:pubmed |
| pubmed-article:11312696 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11312696 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11312696 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11312696 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11312696 | pubmed:issn | 8756-7938 | lld:pubmed |
| pubmed-article:11312696 | pubmed:author | pubmed-author:CloutierCC | lld:pubmed |
| pubmed-article:11312696 | pubmed:author | pubmed-author:BelzileF JFJ | lld:pubmed |
| pubmed-article:11312696 | pubmed:author | pubmed-author:HaffaniY ZYZ | lld:pubmed |
| pubmed-article:11312696 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11312696 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:11312696 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11312696 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11312696 | pubmed:pagination | 211-6 | lld:pubmed |
| pubmed-article:11312696 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:11312696 | pubmed:articleTitle | Bacillus thuringiensis cry3Ca1 protein is toxic to the Colorado potato beetle, Leptinotarsa decemlineata (Say). | lld:pubmed |
| pubmed-article:11312696 | pubmed:affiliation | Département de Phytologie, Pavillon C. E. Marchand, and Département de Biologie, Pavillon Vachon, Université Laval, Sainte-Foy, Québec G1K 7P4, Canada. | lld:pubmed |
| pubmed-article:11312696 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11312696 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
