|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2001-4-23
|
|
pubmed:abstractText |
A specific interaction between the AMPA receptor subunits GluR2 and GluR3 and the fusion protein NSF has recently been identified. Disruption of this interaction by adenoviral-mediated expression of a peptide (pep2m) corresponding to the NSF-binding region of GluR2 results in a dramatic reduction in surface expression of AMPA receptors in primary hippocampal neurons. Here we report that expression of pep2m from a recently developed neuronal-specific adenoviral system gave significant neuroprotection to primary CA1-CA3 hippocampal neurons following stimulation with kainate (KA) and this was accompanied by a reduction in Ca(2+) influx. Protection was also observed following glucose deprivation and exposure to ischemic buffer in the absence of any NMDA receptor antagonists. These results provide strong evidence that AMPA receptors play a direct role in mediating postischemic neurotoxicity.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/N-Ethylmaleimide-Sensitive Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, AMPA,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/glutamate receptor ionotropic...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
1044-7431
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2001 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
17
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
662-70
|
|
pubmed:dateRevised |
2008-12-3
|
|
pubmed:meshHeading |
pubmed-meshheading:11312602-Animals,
pubmed-meshheading:11312602-Brain Ischemia,
pubmed-meshheading:11312602-Calcium,
pubmed-meshheading:11312602-Carrier Proteins,
pubmed-meshheading:11312602-Cell Death,
pubmed-meshheading:11312602-Cells, Cultured,
pubmed-meshheading:11312602-Excitatory Amino Acid Agonists,
pubmed-meshheading:11312602-Gene Expression,
pubmed-meshheading:11312602-Glucose,
pubmed-meshheading:11312602-Hippocampus,
pubmed-meshheading:11312602-Kainic Acid,
pubmed-meshheading:11312602-N-Ethylmaleimide-Sensitive Proteins,
pubmed-meshheading:11312602-Nerve Degeneration,
pubmed-meshheading:11312602-Neurons,
pubmed-meshheading:11312602-Receptors, AMPA,
pubmed-meshheading:11312602-Recombinant Proteins,
pubmed-meshheading:11312602-Transfection,
pubmed-meshheading:11312602-Vesicular Transport Proteins
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Disruption of the GluR2-NSF interaction protects primary hippocampal neurons from ischemic stress.
|
|
pubmed:affiliation |
Division of Medicine, University of Bristol, Marlborough Street, Bristol, BS2 8HW.
|
|
pubmed:publicationType |
Journal Article
|
