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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2001-4-20
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pubmed:abstractText |
Dendritic cells (DCs) efficiently bind and transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV transmission. DC-SIGN, a novel C-type lectin that is expressed in DCs, has recently been shown to bind R5 HIV type 1 (HIV-1) strains and a laboratory-adapted X4 strain. To characterize the interaction of DC-SIGN with primate lentiviruses, we investigated the structural determinants of DC-SIGN required for virus binding and transmission to permissive cells. We constructed a panel of DC-SIGN mutants and established conditions which allowed comparable cell surface expression of all mutants. We found that R5, X4, and R5X4 HIV-1 isolates as well as simian immunodeficiency and HIV-2 strains bound to DC-SIGN and could be transmitted to CD4/coreceptor-positive cell types. DC-SIGN contains a single N-linked carbohydrate chain that is important for efficient cell surface expression but is not required for DC-SIGN-mediated virus binding and transmission. In contrast, C-terminal deletions removing either the lectin binding domain or the repeat region abrogated DC-SIGN function. Trypsin-EDTA treatment inhibited DC-SIGN mediated infection, indicating that virus was maintained at the surface of the DC-SIGN-expressing cells used in this study. Finally, quantitative fluorescence-activated cell sorting analysis of AU1-tagged DC-SIGN revealed that the efficiency of virus transmission was strongly affected by variations in DC-SIGN expression levels. Thus, variations in DC-SIGN expression levels on DCs could greatly affect the susceptibility of human individuals to HIV infection.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10220446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10455052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10508765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10583943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10721994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10721995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10799581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10888639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10891428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-10975799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-11024136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-11226297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-1352913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-1518869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-3472076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-7483259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-7531918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8460475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8629022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8649511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8649512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8658171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8674119,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8849450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8906795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-8906796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-9144465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-9261370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-9300725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-9525605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11312337-9557643
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/DC-specific ICAM-3 grabbing...,
http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Virus,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4664-72
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11312337-Amino Acid Sequence,
pubmed-meshheading:11312337-Animals,
pubmed-meshheading:11312337-Cell Adhesion Molecules,
pubmed-meshheading:11312337-Cell Line, Transformed,
pubmed-meshheading:11312337-Cell Membrane,
pubmed-meshheading:11312337-Edetic Acid,
pubmed-meshheading:11312337-Gene Expression,
pubmed-meshheading:11312337-Glycosylation,
pubmed-meshheading:11312337-HIV-1,
pubmed-meshheading:11312337-HIV-2,
pubmed-meshheading:11312337-Humans,
pubmed-meshheading:11312337-Lectins,
pubmed-meshheading:11312337-Lectins, C-Type,
pubmed-meshheading:11312337-Molecular Sequence Data,
pubmed-meshheading:11312337-Mutagenesis,
pubmed-meshheading:11312337-Receptors, Cell Surface,
pubmed-meshheading:11312337-Receptors, Virus,
pubmed-meshheading:11312337-Simian immunodeficiency virus,
pubmed-meshheading:11312337-Trypsin
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pubmed:year |
2001
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pubmed:articleTitle |
DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus.
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pubmed:affiliation |
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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