Source:http://linkedlifedata.com/resource/pubmed/id/11311058
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2001-4-20
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pubmed:abstractText |
To identify novel peptides that inhibit the interaction between human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120 and CD4, we constructed a targeted phage-displayed peptide library in which phenylalanine and proline were fixed at the fourth and sixth positions, respectively, because Phe43 and the adjacent beta-turn of CD4 are critical for interaction with gp120. Two synthetic peptides were selected after three rounds of biopanning against gp120, and one of them, G1 peptide (ARQPSFDLQCGF), exhibited specific inhibition of the interaction between gp120 and CD4 with an IC(50) of about 50 microM. Structural analysis using NMR demonstrated that G1 peptide forms a compact cyclic structure similar to the CD4 region interacting with gp120. Two derivatives of G1 peptide, a linear hexameric peptide (G1-6) and a cyclic nonameric peptide (G1-c), were synthesized based on the structure of the G1 peptide. Interestingly, they showed higher inhibitory activities than did G1 peptide with IC(50)'s of 6 and 1 microM, respectively. Thus, this study might provide a new insight into the development of anti-HIV-1 inhibitors.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-HIV Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Library
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-2623
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1356-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11311058-Amino Acid Sequence,
pubmed-meshheading:11311058-Anti-HIV Agents,
pubmed-meshheading:11311058-Antigens, CD4,
pubmed-meshheading:11311058-Base Sequence,
pubmed-meshheading:11311058-Coliphages,
pubmed-meshheading:11311058-Crystallography, X-Ray,
pubmed-meshheading:11311058-HIV Envelope Protein gp120,
pubmed-meshheading:11311058-Magnetic Resonance Spectroscopy,
pubmed-meshheading:11311058-Models, Molecular,
pubmed-meshheading:11311058-Molecular Sequence Data,
pubmed-meshheading:11311058-Oligopeptides,
pubmed-meshheading:11311058-Peptide Library,
pubmed-meshheading:11311058-Protein Structure, Secondary
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pubmed:year |
2001
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pubmed:articleTitle |
Short peptides with induced beta-turn inhibit the interaction between HIV-1 gp120 and CD4.
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pubmed:affiliation |
Department of Molecular Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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