Source:http://linkedlifedata.com/resource/pubmed/id/11310824
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2001-4-19
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pubmed:abstractText |
The aberrant content of DNA, or aneuploidy, is a hallmark of tumor cells and may be associated with malignant potential. Based on the hypothesis that aneuploidy, as a form of genetic instability, results in an increased capability to generate cell heterogeneity, we investigated whether a comprehensive assessment of aneuploidy extent and degree might be a reliable indicator of tumor aggressiveness. DNA content was determined by flow cytometry in the infiltrating front of 131 paraffin-embedded primary colorectal carcinomas collected in a prospective design. Enrichment of tumor cells by sample microdissection resulted in neoplastic cell contents above 75%. An estimate of aneuploidy, the aneuploidy index (AI), was calculated as the tumor DNA content adjusted by the percentage of diploid and aneuploid cells in G0/G1. Thirty-nine tumors were diploid, 90 hyperdiploid, and 2 hypodiploid. The mean AI in aneuploid tumors was 1.20+/-0.17 and correlated with Dukes' stage and metastasis (p < 0.05). A high AI (receiver operating characteristic curve cutoff value greater than 1.14) predicted a poorer outcome in univariate (p = 0.004) and multivariate (p = 0.01) analyses. Based on these results, we postulate that aneuploidy is the molecular engine of progression in a subset of colorectal cancers, in which the AI seems to be a sensible and independent gauge of malignant potential. The AI determination may have prognostic application in colorectal cancer, especially in low-grade tumors, which might benefit from coadjuvant therapies.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0023-6837
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
307-15
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11310824-Adult,
pubmed-meshheading:11310824-Aged,
pubmed-meshheading:11310824-Aged, 80 and over,
pubmed-meshheading:11310824-Aneuploidy,
pubmed-meshheading:11310824-Colorectal Neoplasms,
pubmed-meshheading:11310824-DNA, Neoplasm,
pubmed-meshheading:11310824-Female,
pubmed-meshheading:11310824-Flow Cytometry,
pubmed-meshheading:11310824-Humans,
pubmed-meshheading:11310824-Male,
pubmed-meshheading:11310824-Middle Aged,
pubmed-meshheading:11310824-Predictive Value of Tests,
pubmed-meshheading:11310824-Prognosis,
pubmed-meshheading:11310824-Survival Analysis
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pubmed:year |
2001
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pubmed:articleTitle |
Redefining the significance of aneuploidy in the prognostic assessment of colorectal cancer.
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pubmed:affiliation |
Institut de Recerca Oncològica, Hospital Duran i Reynals, L'Hospitalet, Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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