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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-4-18
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pubmed:abstractText |
To detect specific partners of the small Golgi-localized GTPase rab1b we generated rab1b mutants and used them as bait proteins in yeast two-hybrid screens. We isolated several specifically interacting clones. Two of them encode large protein fragments highly homologous to rat GM130 and to human Golgin95. The full-length human GM130 cDNA was cloned and its interaction with rab1b was characterized in detail by yeast two-hybrid and in vitro binding assays. Here we report for the first time that the rab1b protein interacts specifically with GM130 in a GTP-dependent manner and therefore needs the hypervariable regions of the N- and C-termini. We mapped the rab1b binding site of GM130 and provide evidence that it is different to the previously described p115 and Grasp65 binding sites of the GM130 protein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10050856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10209123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10402460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10449330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10493955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10601335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10679020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10720461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10769027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10903204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-10931861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-1429835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-1512287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-1902553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-7615674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-7730399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-7831324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-8163543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-8313902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-8315394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-8521955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-8557739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-8603910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-8918191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9150144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9394917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9490716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9506515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9524117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9545229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9628863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9697774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11306556-9786945
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/GORASP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/GRASP65 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Golgin subfamily A member 2,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RAB1B protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/rab GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rab1 GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/vesicular transport factor p115
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1469-221X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
336-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11306556-Humans,
pubmed-meshheading:11306556-Animals,
pubmed-meshheading:11306556-Rats,
pubmed-meshheading:11306556-Golgi Apparatus,
pubmed-meshheading:11306556-Mutation,
pubmed-meshheading:11306556-Microscopy, Fluorescence,
pubmed-meshheading:11306556-Membrane Proteins,
pubmed-meshheading:11306556-Amino Acid Sequence,
pubmed-meshheading:11306556-Protein Binding,
pubmed-meshheading:11306556-HeLa Cells,
pubmed-meshheading:11306556-Binding Sites,
pubmed-meshheading:11306556-Cell Line,
pubmed-meshheading:11306556-beta-Galactosidase,
pubmed-meshheading:11306556-Models, Genetic,
pubmed-meshheading:11306556-Transformation, Genetic,
pubmed-meshheading:11306556-Molecular Sequence Data,
pubmed-meshheading:11306556-Autoantigens,
pubmed-meshheading:11306556-Carrier Proteins,
pubmed-meshheading:11306556-Protein Structure, Tertiary,
pubmed-meshheading:11306556-Cloning, Molecular,
pubmed-meshheading:11306556-Sequence Homology, Amino Acid,
pubmed-meshheading:11306556-Plasmids,
pubmed-meshheading:11306556-DNA, Complementary,
pubmed-meshheading:11306556-Transfection,
pubmed-meshheading:11306556-Recombinant Proteins,
pubmed-meshheading:11306556-Two-Hybrid System Techniques
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