Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-4-19
pubmed:abstractText
Apoptosis after the loss of cell anchorage--"anoikis"--plays an important role in the life cycle of adherent cells. Furthermore, loss of anchorage dependency is believed to be a critical step in metastatic transformation. The aim of this study was to further characterize the sequence of intracellular events during anoikis in a nontransformed population of human intestinal epithelial cells (IECs). Purified human IECs were kept in suspension to induce anoikis in over 90% of IECs within 3 h. Two initiator caspases, caspase-2 and -9, are activated within 15 min, followed by the hierarchical activation of downstream caspases within 1 h. The activation of the caspase FLICE (caspase-8) does not contribute to the initiation of anoikis, and massive release of cytochrome c from mitochondria cannot be detected before 60 min, indicating that cytochrome c release does not play a role during initiation of anoikis. This study delineates the signaling cascade during anoikis of nontransformed cells. Future studies may identify alterations of this cascade in neoplastic cells, thereby possibly gaining insight into carcinogenesis and metastatic transformation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface, http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CASP6 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CASP7 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CASP8 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CASP9 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 2, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 6, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 7, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 8, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 9, http://linkedlifedata.com/resource/pubmed/chemical/Caspases, http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome c Group
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1044-9523
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11306515-Anoikis, pubmed-meshheading:11306515-Antigens, Surface, pubmed-meshheading:11306515-Caspase 2, pubmed-meshheading:11306515-Caspase 3, pubmed-meshheading:11306515-Caspase 6, pubmed-meshheading:11306515-Caspase 7, pubmed-meshheading:11306515-Caspase 8, pubmed-meshheading:11306515-Caspase 9, pubmed-meshheading:11306515-Caspases, pubmed-meshheading:11306515-Cell Adhesion, pubmed-meshheading:11306515-Cell Communication, pubmed-meshheading:11306515-Cells, Cultured, pubmed-meshheading:11306515-Colon, pubmed-meshheading:11306515-Cytochrome c Group, pubmed-meshheading:11306515-Epithelial Cells, pubmed-meshheading:11306515-Humans, pubmed-meshheading:11306515-Immunohistochemistry, pubmed-meshheading:11306515-Intestinal Mucosa, pubmed-meshheading:11306515-Protein Transport, pubmed-meshheading:11306515-Signal Transduction
pubmed:year
2001
pubmed:articleTitle
Apoptotic signaling during initiation of detachment-induced apoptosis ("anoikis") of primary human intestinal epithelial cells.
pubmed:affiliation
Department of Medicine I, University of Regensburg, Germany. Johannes.Grossmann@klinik.uni-regensburg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't