Source:http://linkedlifedata.com/resource/pubmed/id/11303723
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-4-16
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| pubmed:abstractText |
Among identified adhesion molecules, the L-selectin on neutrophils enables the first step of leukocyte adherence to activated endothelial cells. To allow firm adhesion of neutrophils, L-selectin is then split off the cell membrane. It was hypothetized that an increase of the constitutively high serum level of soluble L-selectin may indicate an ongoing pathological neutrophil sequestration to the endothelial cells associated with activation and injury of the cells. To evaluate this hypothesis, sL-selectin serum levels and neutrophil L-selectin expression of healthy volunteers (group A, n = 15), as well as of surgical patients, were investigated. Group B (n = 26) included patients subjected to elective limb surgery (mean operation time, 122 min), and group C (n = 45) comprised trauma patients. sL-selectin serum levels were measured daily over a 14-day period. Neutrophil L-selectin expression was evaluated by FACS analysis using the humanized anti-L-selectin antibody HuDreg 55 over a period of 3 days at minimum in both experimental groups. The binding of sL-selectin to endothelial cells was also examined in vitro. Elective limb surgery resulted in lower pre- and post-operative sL-selectin plasma levels (800-1,000 ng/mL) compared to healthy volunteers (1,100-1,200 ng/mL) with insignificant changes throughout the study period. Trauma patients revealed even lower sL-selectin levels (400-600 ng/mL). When these patients were discriminated by the multiple organ dysfunction (MOD) score of Moore in +MOD (n = 9, ISS = 31.7) and -MOD (n = 36, ISS = 25.0), a significant difference became evident. In +MOD patients sL-selectin levels remained on a low basis of 350 ng/mL, whereas in -MOD patients the initial low sL-selectin level subsequently rose to 800 ng/mL, similar to that of elective surgery patients. FACS analysis revealed a significant drop in neutrophil L-selectin expression 24 h after trauma compared to normal. Also, +MOD and -MOD patients were significantly discriminated by the L-selectin expression at this time. The in vitro studies revealed evidence for binding of sL-selectin to endothelial cells independently on the presence of neutrophils. According to our data, increasing severity of the post-operative/posttraumatic course is associated with decreasing sL-selectin serum levels and also reduced neutrophil L-selectin expression. In view of the in vitro results, this probably indicates competitive enhanced binding of sL-selectin to endothelial cells, thus masking the elevated activation of neutrophils and their ability for endothelial adherence.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1073-2322
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
15
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
254-60
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11303723-Adolescent,
pubmed-meshheading:11303723-Adult,
pubmed-meshheading:11303723-Cell Adhesion,
pubmed-meshheading:11303723-Cells, Cultured,
pubmed-meshheading:11303723-Chemotaxis, Leukocyte,
pubmed-meshheading:11303723-Endothelium, Vascular,
pubmed-meshheading:11303723-Flow Cytometry,
pubmed-meshheading:11303723-Gene Expression Regulation,
pubmed-meshheading:11303723-Humans,
pubmed-meshheading:11303723-L-Selectin,
pubmed-meshheading:11303723-Middle Aged,
pubmed-meshheading:11303723-Multiple Organ Failure,
pubmed-meshheading:11303723-Multiple Trauma,
pubmed-meshheading:11303723-Neutrophils,
pubmed-meshheading:11303723-Prospective Studies,
pubmed-meshheading:11303723-Recombinant Proteins,
pubmed-meshheading:11303723-Severity of Illness Index,
pubmed-meshheading:11303723-Solubility,
pubmed-meshheading:11303723-Surgical Procedures, Elective,
pubmed-meshheading:11303723-Wounds and Injuries
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| pubmed:year |
2001
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| pubmed:articleTitle |
The effect of trauma on neutrophil L-selectin expression and sL-selectin serum levels.
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| pubmed:affiliation |
Unfallchirurgische Klinik, Medizinische Hochschule Hannover, FRG.
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| pubmed:publicationType |
Journal Article,
Comparative Study
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