Source:http://linkedlifedata.com/resource/pubmed/id/11302520
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2001-4-13
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| pubmed:abstractText |
Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1and the Pancreatitis Associated Protein I (PAP I) are concomitantly expressed in the epithelial cells of the lower part of the intestinal crypts. Because Cdx1 is a transcription factor and PAP I, in other tissues, is a proliferative factor, we looked for a relationship between these two proteins in the intestinal-derived IEC-6 cells. After stable transfection with a Cdx1 expression vector, they produce high levels of the PAP I transcript and protein indicating a functional link between the two genes. Demonstration of Cdx1 binding to the PAP I promoter region and suppression of PAP I induction after deletion of the corresponding sequence indicated that Cdx1 is a transcription factor controlling PAP I gene expression in intestinal cells. By infecting IEC-6 cells with adenoviruses expressing PAP I, we demonstrated that PAP I induces mitosis in these cells. On the other hand, inhibition of the PAP I expression in the IEC-6 Cdxl-expressing cells using an antisense strategy confirmed the requirement of this protein for the effect of Cdx1 on cell growth. Finally, addition of the immunopurified PAP I to the culture medium promotes cell growth of the IEC-6 cells in a dose-dependent manner. Maximal effect was obtained at 1 ng/ml. Taken together these results demonstrate that PAP I is a target of the Cdx1 homeobox gene in intestinal cells which participates in the regulation of intestinal cell growth via an autocrine and/or paracrine mechanism.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acute-Phase Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/CDX1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cdx1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/pancreatitis-associated protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0171-9335
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
80
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
156-63
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| pubmed:dateRevised |
2010-2-8
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| pubmed:meshHeading |
pubmed-meshheading:11302520-Acute-Phase Proteins,
pubmed-meshheading:11302520-Animals,
pubmed-meshheading:11302520-Antigens, Neoplasm,
pubmed-meshheading:11302520-Cell Division,
pubmed-meshheading:11302520-Cell Line,
pubmed-meshheading:11302520-Epithelial Cells,
pubmed-meshheading:11302520-Gene Expression Regulation,
pubmed-meshheading:11302520-Homeodomain Proteins,
pubmed-meshheading:11302520-Humans,
pubmed-meshheading:11302520-Intestinal Mucosa,
pubmed-meshheading:11302520-Lectins, C-Type,
pubmed-meshheading:11302520-Mitosis,
pubmed-meshheading:11302520-Promoter Regions, Genetic,
pubmed-meshheading:11302520-Rats,
pubmed-meshheading:11302520-Response Elements,
pubmed-meshheading:11302520-Transcription Factors,
pubmed-meshheading:11302520-Transcriptional Activation,
pubmed-meshheading:11302520-Tumor Markers, Biological
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| pubmed:year |
2001
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| pubmed:articleTitle |
Cdx1 promotes cellular growth of epithelial intestinal cells through induction of the secretory protein PAP I.
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| pubmed:affiliation |
Laboratoire de Recherche de Physiologie et Pathologie Digestives, INSERM U.315, Marseille, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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