Source:http://linkedlifedata.com/resource/pubmed/id/11297622
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2001-4-12
|
| pubmed:abstractText |
Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) possesses both growth-inhibitory and -potentiating effects on cells that are independent of IGF action and are mediated through specific IGFBP-3 binding proteins/receptors located at the cell membrane, cytosol, or nuclear compartments and in the extracellular matrix. We have here characterized transferrin (Tf) as one of these IGFBP-3 binding proteins. Human serum was fractionated over an IGFBP-3 affinity column, and a 70-kDa protein was eluted, sequenced, and identified (through database searching and Western immunoblot) as human Tf. Tf bound IGFBP-3 but had negligible affinity to the other five IGFBPs, and iron-saturated holo-Tf bound IGFBP-3 more avidly than unsaturated Tf. Biosensor interaction analysis confirmed that this interaction is specific and sensitive, with a high association rate similar to IGF-I, and suggested that binding occurs in the vicinity of the IGFBP-3 nuclear localization site. As an independent confirmation of this interaction, using a yeast two-hybrid system, we cloned Tf from a human liver complementary DNA library as an IGFBP-3 protein partner. Tf treatment blocked IGFBP-3-induced cell proliferation in bladder smooth muscle cells, and IGFBP-3-induced apoptosis in prostate cancer cells. In summary, we have employed a combination of techniques to demonstrate that Tf specifically binds IGFBP-3, and we showed that this interaction has important physiological effects on cellular events.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Iron,
http://linkedlifedata.com/resource/pubmed/chemical/Transferrin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0021-972X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
86
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1806-13
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11297622-Adult,
pubmed-meshheading:11297622-Animals,
pubmed-meshheading:11297622-Apoptosis,
pubmed-meshheading:11297622-Carrier Proteins,
pubmed-meshheading:11297622-Cell Division,
pubmed-meshheading:11297622-Cells, Cultured,
pubmed-meshheading:11297622-Dose-Response Relationship, Drug,
pubmed-meshheading:11297622-Fluorescent Antibody Technique,
pubmed-meshheading:11297622-Humans,
pubmed-meshheading:11297622-Insulin-Like Growth Factor Binding Protein 3,
pubmed-meshheading:11297622-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:11297622-Iron,
pubmed-meshheading:11297622-Kinetics,
pubmed-meshheading:11297622-Male,
pubmed-meshheading:11297622-Microscopy, Confocal,
pubmed-meshheading:11297622-Muscle, Smooth,
pubmed-meshheading:11297622-Prostatic Neoplasms,
pubmed-meshheading:11297622-Sheep,
pubmed-meshheading:11297622-Tissue Distribution,
pubmed-meshheading:11297622-Transferrin,
pubmed-meshheading:11297622-Urinary Bladder,
pubmed-meshheading:11297622-Yeasts
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| pubmed:year |
2001
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| pubmed:articleTitle |
Transferrin is an insulin-like growth factor-binding protein-3 binding protein.
|
| pubmed:affiliation |
Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|