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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
29
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pubmed:dateCreated |
2001-7-16
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pubmed:databankReference |
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pubmed:abstractText |
We have characterized platelet-derived growth factor (PDGF) C, a novel growth factor belonging to the PDGF family. PDGF-C is a multidomain protein with the N-terminal region homologous to the extracellular CUB domain of neuropilin-1, and the C-terminal region consists of a growth factor domain (GFD) with homology to vascular endothelial growth factor (25%) and PDGF A-chain (23%). A serum-sensitive cleavage site between the two domains allows release of the GFD from the CUB domain. Competition binding and immunoprecipitation studies on cells bearing both PDGF alpha and beta receptors reveal a high affinity binding of recombinant GFD (PDGF-CC) to PDGF receptor-alpha homodimers and PDGF receptor-alpha/beta heterodimers. PDGF-CC exhibits greater mitogenic potency than PDGF-AA and comparable or greater mitogenic activity than PDGF-AB and PDGF-BB on several mesenchymal cell types. Analysis of PDGF-CC in vivo in a diabetic mouse model of delayed wound healing showed that PDGF-CC significantly enhanced repair of a full-thickness skin excision. Together, these studies describe a third member of the PDGF family (PDGF-C) as a potent mitogen for cells of mesenchymal origin in in vitro and in vivo systems with a binding pattern similar to PDGF-AB.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Platelet-Derived Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Platelet-Derived Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/platelet-derived growth factor C
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:BukowskiT RTR,
pubmed-author:DuffM EME,
pubmed-author:FeldhausA LAL,
pubmed-author:GilbertsonD GDG,
pubmed-author:HEL,
pubmed-author:HartC ECE,
pubmed-author:HofstrandP DPD,
pubmed-author:HumesJ MJM,
pubmed-author:KellyJ DJD,
pubmed-author:MooreMM,
pubmed-author:PalmerT ETE,
pubmed-author:SheppardP OPO,
pubmed-author:ShoemakerKK,
pubmed-author:WestJ WJW
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27406-14
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11297552-Amino Acid Sequence,
pubmed-meshheading:11297552-Animals,
pubmed-meshheading:11297552-Aorta,
pubmed-meshheading:11297552-Cell Line,
pubmed-meshheading:11297552-Cricetinae,
pubmed-meshheading:11297552-DNA, Complementary,
pubmed-meshheading:11297552-Diabetes Mellitus, Experimental,
pubmed-meshheading:11297552-Humans,
pubmed-meshheading:11297552-Lymphokines,
pubmed-meshheading:11297552-Mice,
pubmed-meshheading:11297552-Molecular Sequence Data,
pubmed-meshheading:11297552-Platelet-Derived Growth Factor,
pubmed-meshheading:11297552-Protein Binding,
pubmed-meshheading:11297552-Receptor, Platelet-Derived Growth Factor alpha,
pubmed-meshheading:11297552-Receptor, Platelet-Derived Growth Factor beta,
pubmed-meshheading:11297552-Recombinant Proteins,
pubmed-meshheading:11297552-Sequence Homology, Amino Acid,
pubmed-meshheading:11297552-Thymidine,
pubmed-meshheading:11297552-Wound Healing
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pubmed:year |
2001
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pubmed:articleTitle |
Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor.
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pubmed:affiliation |
ZymoGenetics Inc., Seattle, Washington 98102, USA. gilbertd@zgi.com
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pubmed:publicationType |
Journal Article
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