Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-4-11
pubmed:abstractText
The existence of distinct tubulins in microtubules forming the sperm axoneme has been demonstrated in various species, whereas little is known about the distribution of tubulin variants in insect spermatozoa. In the present study, a panel of specific antibodies has been used to investigate the presence and localization of tubulin isotypes and post-translationally modified tubulins in the spermatozoon of the stick insect Bacillus rossius. Indirect immunofluorescence and immunogold staining showed differences in labelling in the mature sperm and that the tubulin epitopes localized differentially in the axoneme. In particular, the tyrosinated alpha-tubulin mainly occurs on doublets. These results provide an insight into the molecular composition of the microtubules forming the sperm axoneme of B. rossius and suggest that the structural specificity could reflect distinct functional roles within axonemal microtubules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1122-9497
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
635-43
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Immunolocalization of different tubulin epitopes in the spermatozoon of Bacillus rossius (Insecta, Phasmatodea).
pubmed:affiliation
Department of Environmental Sciences, University of Tuscia, Viterbo, Italy. artaddei@unitus.it
pubmed:publicationType
Journal Article