|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2001-4-11
|
|
pubmed:abstractText |
Backbone modifications have been introduced into the melanoma derived peptide MART-1(27-35) to increase its binding to class I major histocompatibility complex HLA-A2 molecule, and ultimately to enhance its immunogenicity. Each analogue was obtained by replacing one peptide bond at a time in the natural epitope by the aminomethylene (CH2-NH) surrogate. All analogues displayed an increased resistance to proteolysis. Interestingly, the comparative results showed that five analogues bound more efficiently to HLA-A2 than the parent peptide. On the other hand, two pseudopeptide/HLA-A2 complexes were recognized by one melanoma-specific T cell clone. Close examination of the impact of such modifications at the molecular level provides useful supports for the rational design of stable compounds with applications in anti-tumour specific immunotherapy and in vaccine development.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
1075-2617
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
7
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
157-65
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11297352-Amino Acid Sequence,
pubmed-meshheading:11297352-Amino Acids,
pubmed-meshheading:11297352-Cell Line,
pubmed-meshheading:11297352-Chromatography, High Pressure Liquid,
pubmed-meshheading:11297352-Dose-Response Relationship, Drug,
pubmed-meshheading:11297352-Epitopes,
pubmed-meshheading:11297352-Fluorenes,
pubmed-meshheading:11297352-HLA-A2 Antigen,
pubmed-meshheading:11297352-Humans,
pubmed-meshheading:11297352-Melanoma,
pubmed-meshheading:11297352-Molecular Sequence Data,
pubmed-meshheading:11297352-Neoplasm Proteins,
pubmed-meshheading:11297352-Peptides,
pubmed-meshheading:11297352-Protein Binding,
pubmed-meshheading:11297352-T-Lymphocytes
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Synthesis and antigenic properties of reduced peptide bond analogues of an immunodominant epitope of the melanoma MART-1 protein.
|
|
pubmed:affiliation |
Institut de Biologie Moléculaire et Cellulaire, UPR 9021-CNRS, Strasbourg, France.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
