Linked Life Data

11294846

Source:http://linkedlifedata.com/resource/pubmed/id/11294846

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
2001-6-18
pubmed:abstractText
Renal mesangial cells regulate their expression of the pro-inflammatory gene cyclooxygenase-2 (COX-2) through mechanisms involving gene transcription and post-transcriptional events. Post-transcriptional regulation of COX-2 is dependent, in part, on sequences within the 3'-untranslated region (3'-UTR) of the COX-2 mRNA. Insertion of the entire 3'-UTR of COX-2 into the 3'-UTR of luciferase resulted in a 70% decrease in luciferase enzymatic activity. Measurement of steady-state reporter gene mRNA levels suggested that the loss of activity was due to decreased translational efficiency. Deletion analysis identified the first 60 nucleotides of the 3'-UTR of COX-2 as a major translational control element. This region of the 3'-UTR of COX-2 is highly conserved across species; is AU-rich; and contains multiple repeats of the regulatory sequence AUUUA, reported to confer post-transcriptional control. In addition, we identified regions of the 3'-UTR of COX-2 outside of the first 60 nucleotides that altered message stability. Some of these regions contained AUUUA consensus sequences, whereas others did not, and represent novel control elements. These results suggest that expression of COX-2 in mesangial cells depends on the complex integration of multiple signals derived from the 3'-UTR of the message.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23179-85
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
The 3'-untranslated region of murine cyclooxygenase-2 contains multiple regulatory elements that alter message stability and translational efficiency.
pubmed:affiliation
Departments of Medicine and of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.