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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2001-4-10
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pubmed:abstractText |
Cysteine string protein (Csp) is a secretory vesicle protein previously demonstrated to be required for Ca2+-regulated exocytosis in neurons and endocrine cells. It has been suggested to function by regulating voltage-gated Ca2+ channels or, alternatively, to have a more direct effect on the regulated exocytotic machinery. Here we demonstrate the expression of Csp in mammary epithelial cells and in the KIM-2 mammary cell line. In KIM-2 cells, Csp was found to be associated with a population of small vesicles and showed partial co-distribution with the vesicle protein cellubrevin. KIM-2 cells do not express detectable levels of voltage-gated Ca2+ channels, ruling these out as a site of action. Using the release of transfected growth hormone (GH) as an assay of secretion, we found that GH is secreted in an exclusively constitutive manner from KIM-2 cells. Overexpression of Csp1 inhibits regulated exocytosis in other cell types but has no effect on constitutive GH release by KIM-2 cells. These results suggest that Csp does not have a major function in constitutive exocytosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0031-6768
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
441
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
639-49
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11294245-Animals,
pubmed-meshheading:11294245-Antisense Elements (Genetics),
pubmed-meshheading:11294245-Cell Line, Transformed,
pubmed-meshheading:11294245-Epithelial Cells,
pubmed-meshheading:11294245-Exocytosis,
pubmed-meshheading:11294245-Female,
pubmed-meshheading:11294245-Gene Expression,
pubmed-meshheading:11294245-Growth Hormone,
pubmed-meshheading:11294245-HSP40 Heat-Shock Proteins,
pubmed-meshheading:11294245-Lactation,
pubmed-meshheading:11294245-Mammary Glands, Animal,
pubmed-meshheading:11294245-Membrane Potentials,
pubmed-meshheading:11294245-Membrane Proteins,
pubmed-meshheading:11294245-Mice,
pubmed-meshheading:11294245-Mice, Transgenic,
pubmed-meshheading:11294245-Patch-Clamp Techniques,
pubmed-meshheading:11294245-Protein Transport,
pubmed-meshheading:11294245-RNA, Messenger,
pubmed-meshheading:11294245-Secretory Vesicles,
pubmed-meshheading:11294245-Transfection,
pubmed-meshheading:11294245-Vesicle-Associated Membrane Protein 3
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pubmed:year |
2001
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pubmed:articleTitle |
Cysteine string protein expression in mammary epithelial cells.
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pubmed:affiliation |
The Physiological Laboratory, University of Liverpool, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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