| pubmed-article:11292702 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C0009184 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C0009186 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C0041945 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:11292702 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:11292702 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:11292702 | pubmed:dateCreated | 2001-4-9 | lld:pubmed |
| pubmed-article:11292702 | pubmed:abstractText | Coccidioides immitis antigens which stimulate a T helper cell 1 (Th1) pathway of host immune response are considered to be essential components of a vaccine against coccidioidomycosis. Recombinant urease (rURE) and recombinant heat shock protein 60 (rHSP60) of C. immitis were expressed in Escherichia coli and tested as vaccine candidates in BALB/c mice. A synthetic oligodeoxynucleotide which contained unmethylated CpG dinucleotides and was previously shown to enhance a murine Th1 response was used as an immunoadjuvant. T cells isolated from the spleens and lymph nodes of the rURE- and rHSP60-immune mice showed in vitro proliferative responses to the respective recombinant protein, but only those T lymphocytes from rURE-immunized mice revealed markedly elevated levels of expression of selected Th1-type cytokine genes. BALB/c mice immunized subcutaneously with rURE and subsequently challenged by the intraperitoneal (i.p.) route with a lethal inoculum of C. immitis arthroconidia demonstrated a significant reduction in the level of C. immitis infection compared to control animals. rHSP60 was much less effective as a protective antigen. Evaluation of cytokine gene expression in lung tissue and levels of recombinant urease-specific immunoglobulins (immunoglobulin G1 [IgG1] versus IgG2a) in murine sera at 12 days after challenge provided additional evidence that immunization with rURE stimulated a Th1 response to the pathogen. Urease was further evaluated by expression of the URE gene in a mammalian plasmid vector (pSecTag2A.URE) which was used to immunize mice by the intradermal route. In this case, 82% of the vector construct-immunized animals survived more than 40 days after i.p. infection, compared to only 10% of the mice immunized with the vector alone. In addition, 87% of the pSecTag2A.URE-immunized survivors had sterile lungs and spleens. These data support the need for further evaluation of the C. immitis urease as a candidate vaccine against coccidioidomycosis. | lld:pubmed |
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| pubmed-article:11292702 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11292702 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292702 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11292702 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11292702 | pubmed:month | May | lld:pubmed |
| pubmed-article:11292702 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:11292702 | pubmed:author | pubmed-author:LiKK | lld:pubmed |
| pubmed-article:11292702 | pubmed:author | pubmed-author:HuntC FCF | lld:pubmed |
| pubmed-article:11292702 | pubmed:author | pubmed-author:LehmannP FPF | lld:pubmed |
| pubmed-article:11292702 | pubmed:author | pubmed-author:ColeG TGT | lld:pubmed |
| pubmed-article:11292702 | pubmed:author | pubmed-author:YuJ JJJ | lld:pubmed |
| pubmed-article:11292702 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11292702 | pubmed:volume | 69 | lld:pubmed |
| pubmed-article:11292702 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11292702 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11292702 | pubmed:pagination | 2878-87 | lld:pubmed |
| pubmed-article:11292702 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11292702 | pubmed:meshHeading | pubmed-meshheading:11292702... | lld:pubmed |
| pubmed-article:11292702 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11292702 | pubmed:articleTitle | Recombinant urease and urease DNA of Coccidioides immitis elicit an immunoprotective response against coccidioidomycosis in mice. | lld:pubmed |
| pubmed-article:11292702 | pubmed:affiliation | Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 43614-5806, USA. | lld:pubmed |
| pubmed-article:11292702 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11292702 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11292702 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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