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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2001-4-9
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pubmed:abstractText |
Coccidioides immitis antigens which stimulate a T helper cell 1 (Th1) pathway of host immune response are considered to be essential components of a vaccine against coccidioidomycosis. Recombinant urease (rURE) and recombinant heat shock protein 60 (rHSP60) of C. immitis were expressed in Escherichia coli and tested as vaccine candidates in BALB/c mice. A synthetic oligodeoxynucleotide which contained unmethylated CpG dinucleotides and was previously shown to enhance a murine Th1 response was used as an immunoadjuvant. T cells isolated from the spleens and lymph nodes of the rURE- and rHSP60-immune mice showed in vitro proliferative responses to the respective recombinant protein, but only those T lymphocytes from rURE-immunized mice revealed markedly elevated levels of expression of selected Th1-type cytokine genes. BALB/c mice immunized subcutaneously with rURE and subsequently challenged by the intraperitoneal (i.p.) route with a lethal inoculum of C. immitis arthroconidia demonstrated a significant reduction in the level of C. immitis infection compared to control animals. rHSP60 was much less effective as a protective antigen. Evaluation of cytokine gene expression in lung tissue and levels of recombinant urease-specific immunoglobulins (immunoglobulin G1 [IgG1] versus IgG2a) in murine sera at 12 days after challenge provided additional evidence that immunization with rURE stimulated a Th1 response to the pathogen. Urease was further evaluated by expression of the URE gene in a mammalian plasmid vector (pSecTag2A.URE) which was used to immunize mice by the intradermal route. In this case, 82% of the vector construct-immunized animals survived more than 40 days after i.p. infection, compared to only 10% of the mice immunized with the vector alone. In addition, 87% of the pSecTag2A.URE-immunized survivors had sterile lungs and spleens. These data support the need for further evaluation of the C. immitis urease as a candidate vaccine against coccidioidomycosis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-10068388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-10338502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-10417129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-10639421,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-13761479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-14253511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-1717559,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-1987047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-3102379,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-7642285,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-9573127,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-9673228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-9712793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11292702-9916069
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Chaperonin 60,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Urease,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2878-87
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11292702-Animals,
pubmed-meshheading:11292702-Antibodies, Fungal,
pubmed-meshheading:11292702-Chaperonin 60,
pubmed-meshheading:11292702-Coccidioides,
pubmed-meshheading:11292702-Coccidioidomycosis,
pubmed-meshheading:11292702-Cytokines,
pubmed-meshheading:11292702-Female,
pubmed-meshheading:11292702-Fungal Vaccines,
pubmed-meshheading:11292702-Immunization,
pubmed-meshheading:11292702-Lymphocyte Activation,
pubmed-meshheading:11292702-Mice,
pubmed-meshheading:11292702-Mice, Inbred BALB C,
pubmed-meshheading:11292702-Recombinant Proteins,
pubmed-meshheading:11292702-T-Lymphocytes,
pubmed-meshheading:11292702-Urease,
pubmed-meshheading:11292702-Vaccines, DNA,
pubmed-meshheading:11292702-Vaccines, Synthetic
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pubmed:year |
2001
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pubmed:articleTitle |
Recombinant urease and urease DNA of Coccidioides immitis elicit an immunoprotective response against coccidioidomycosis in mice.
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pubmed:affiliation |
Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 43614-5806, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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