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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2001-4-6
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pubmed:abstractText |
We investigated the roles of estrogens and androgens in the progesterone biosynthesis of bovine luteal cells. The responsiveness of primary luteal cells to the stimulation of tropic agents was observed in a dose-dependent manner. Estrogens and androgens significantly inhibited tropic agent-induced progesterone secretions, but glucocorticoids did not, which indicated the inhibitions were specific. The failure of exogenous 8-Br-cAMP to prevent these inhibitions suggested that took place at the post-cAMP steps. The immunoblot showed that testosterone remarkably decreased the amount of induced P450scc protein after 6-hour treatment, yet 17beta-estradiol did not. The 3beta-HSD activity assays demonstrated that both 17beta-estradiol and testosterone efficiently blocked induced 3beta-HSD activities. Both inhibitory effects of E2 and T on progesterone synthesis were observed one hour after treatment and accompanied with suppressed 3beta-HSD activities. This study presents that estrogens and androgens specifically inhibit bovine luteal function through different mechanisms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1851-65
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11292063-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:11292063-Animals,
pubmed-meshheading:11292063-Cattle,
pubmed-meshheading:11292063-Cells, Cultured,
pubmed-meshheading:11292063-Cholesterol Side-Chain Cleavage Enzyme,
pubmed-meshheading:11292063-Corpus Luteum,
pubmed-meshheading:11292063-Dose-Response Relationship, Drug,
pubmed-meshheading:11292063-Enzyme Inhibitors,
pubmed-meshheading:11292063-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11292063-Estradiol,
pubmed-meshheading:11292063-Female,
pubmed-meshheading:11292063-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:11292063-Immunoblotting,
pubmed-meshheading:11292063-Progesterone,
pubmed-meshheading:11292063-Testosterone
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pubmed:year |
2001
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pubmed:articleTitle |
Differential inhibition of progesterone synthesis in bovine luteal cells by estrogens and androgens.
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pubmed:affiliation |
Department of Veterinary Medicine, National Taiwan University, Taipei, Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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