Source:http://linkedlifedata.com/resource/pubmed/id/11290598
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2001-4-6
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| pubmed:abstractText |
We have previously shown that CD4(+) T cell-mediated allergic inflammation is diminished in signal transducer and activator of transcription (Stat)5a-deficient (Stat5a(-/-)) mice. To determine whether Stat5a regulates T helper cell differentiation, we studied T helper (Th)1 and Th2 cell differentiation of Stat5a(-/-)CD4(+) T cells at single-cell levels. First, Th2 cell differentiation from antigen-stimulated splenocytes was significantly decreased in Stat5a(-/-) mice as compared with that in wild-type mice. Further, Th2 cell differentiation was also impaired in Stat5a(-/-) mice even when purified CD4(+) T cells were stimulated with anti-CD3 plus anti-CD28 antibodies in the presence of interleukin-4. Moreover, the retrovirus-mediated gene expression of Stat5a in Stat5a(-/-)CD4(+) T cells restored the Th2 cell differentiation at the similar levels to that in wild-type CD4(+) T cells. In addition, interleukin-4 normally phosphorylated Stat6 in CD4(+) T cells from Stat5a(-/-) mice. Second, the development of CD4(+)CD25(+) immunoregulatory T cells was impaired in Stat5a(-/-) mice, as indicated by a significant decrease in the number of CD4(+)CD25(+) T cells in Stat5a(-/-) mice. Furthermore, the depletion of CD4(+)CD25(+) T cells from wild-type splenocytes significantly decreased Th2 cell differentiation but increased Th1 cell differentiation, whereas the depletion of CD4(+)CD25(+) T cells from Stat5a(-/-) splenocytes had no significant effect on the Th1 and Th2 cell differentiation. Together, these results indicate that the intrinsic expression of Stat5a in CD4(+) T cells is required for Th2 cell differentiation and that Stat5a is involved in the development of CD4(+)CD25(+) immunoregulatory T cells that modulate T helper cell differentiation toward Th2 cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat5a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Stat6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
97
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2358-65
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11290598-Animals,
pubmed-meshheading:11290598-Antigens, CD4,
pubmed-meshheading:11290598-Cell Cycle,
pubmed-meshheading:11290598-Cell Differentiation,
pubmed-meshheading:11290598-DNA-Binding Proteins,
pubmed-meshheading:11290598-Gene Expression,
pubmed-meshheading:11290598-Interleukin-4,
pubmed-meshheading:11290598-Mice,
pubmed-meshheading:11290598-Mice, Inbred C57BL,
pubmed-meshheading:11290598-Mice, Knockout,
pubmed-meshheading:11290598-Mice, Transgenic,
pubmed-meshheading:11290598-Milk Proteins,
pubmed-meshheading:11290598-Receptors, Interleukin-2,
pubmed-meshheading:11290598-Recombinant Fusion Proteins,
pubmed-meshheading:11290598-STAT5 Transcription Factor,
pubmed-meshheading:11290598-STAT6 Transcription Factor,
pubmed-meshheading:11290598-Specific Pathogen-Free Organisms,
pubmed-meshheading:11290598-Spleen,
pubmed-meshheading:11290598-Th1 Cells,
pubmed-meshheading:11290598-Th2 Cells,
pubmed-meshheading:11290598-Trans-Activators
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| pubmed:year |
2001
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| pubmed:articleTitle |
Stat5a regulates T helper cell differentiation by several distinct mechanisms.
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| pubmed:affiliation |
Department of Internal Medicine II, Chiba University School of Medicine, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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