Source:http://linkedlifedata.com/resource/pubmed/id/11290506
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2001-4-6
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| pubmed:abstractText |
Epithelial cells lining the airways are thought to play a prominent role in respiratory diseases. We utilized cDNA representational difference analysis to identify the genes in which expression is induced by the proinflammatory cytokines tumor necrosis factor-alpha and interleukin-1beta in primary human bronchial epithelial cells and hence are relevant to airway inflammation. Hybridization of the subtraction product to arrayed cDNAs indicated that known tumor necrosis factor-alpha- and interleukin-1beta-inducible genes such as B94, Zfp36, and regulated on activation normal T cell expressed and secreted were represented, confirming the success of the subtraction experiment. A 1,152-clone library potentially representing genes with higher transcript levels in cytokine-treated human bronchial epithelial cells was generated and sequenced. Sequence similarity searches indicated that these clones represented 57 genes of known function, 1 gene of unknown function, 6 expressed sequence tags, and 2 novel sequences. The expression of 19 of these clones was studied by a combination of Northern blotting and RT-PCR analyses and confirmation of differential expression for 10 known genes, 2 expressed sequence tags, and a novel sequence not represented in any of the public databases was obtained. Thus cDNA representational difference analysis was utilized to isolate known and novel differentially expressed genes, which putatively play a role in airway inflammation.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1040-0605
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
280
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
L841-52
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11290506-Blotting, Northern,
pubmed-meshheading:11290506-Bronchi,
pubmed-meshheading:11290506-Cells, Cultured,
pubmed-meshheading:11290506-Cytokines,
pubmed-meshheading:11290506-DNA, Complementary,
pubmed-meshheading:11290506-Epithelial Cells,
pubmed-meshheading:11290506-Expressed Sequence Tags,
pubmed-meshheading:11290506-Gene Expression Profiling,
pubmed-meshheading:11290506-Humans,
pubmed-meshheading:11290506-Inflammation Mediators,
pubmed-meshheading:11290506-Interleukin-1,
pubmed-meshheading:11290506-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:11290506-Organ Specificity,
pubmed-meshheading:11290506-Pneumonia,
pubmed-meshheading:11290506-Respiratory Mucosa,
pubmed-meshheading:11290506-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11290506-Sequence Analysis, DNA,
pubmed-meshheading:11290506-Tumor Necrosis Factor-alpha
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| pubmed:year |
2001
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| pubmed:articleTitle |
Identification of genes induced by inflammatory cytokines in airway epithelium.
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| pubmed:affiliation |
Novartis Horsham Research Centre, Horsham RH12 5AB, United Kingdom.
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| pubmed:publicationType |
Journal Article
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