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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-4-6
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pubmed:abstractText |
HIV causes a chronic infection by evading immune eradication. A key element of HIV immune escape is the HIV-1 Nef protein. Nef causes a reduction in the level of cell surface major histocompatibility complex class I (MHC-I) protein expression, thus protecting HIV-infected cells from anti-HIV cytotoxic T lymphocyte (CTL) recognition and killing. Nef also reduces cell surface levels of the HIV receptor, CD4, by accelerating endocytosis. We show here that endocytosis is not required for Nef-mediated downmodulation of MHC-I molecules. The main effect of Nef is to block transport of MHC-I molecules to the cell surface, leading to accumulation in intracellular organelles. Furthermore, the effect of Nef on MHC-I molecules (but not on CD4) requires phosphoinositide 3-kinase (PI 3-kinase) activity. We propose that Nef diverts MHC-1 proteins into a PI 3-kinase-dependent transport pathway that prevents expression on the cell surface.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Chromones,
http://linkedlifedata.com/resource/pubmed/chemical/Dynamins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP Phosphohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, nef,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Morpholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/nef Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
267-77
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11289809-Antigens, CD4,
pubmed-meshheading:11289809-Blotting, Western,
pubmed-meshheading:11289809-Cell Membrane,
pubmed-meshheading:11289809-Chromones,
pubmed-meshheading:11289809-Down-Regulation,
pubmed-meshheading:11289809-Dynamins,
pubmed-meshheading:11289809-Endocytosis,
pubmed-meshheading:11289809-Flow Cytometry,
pubmed-meshheading:11289809-GTP Phosphohydrolases,
pubmed-meshheading:11289809-Gene Products, nef,
pubmed-meshheading:11289809-Golgi Apparatus,
pubmed-meshheading:11289809-HIV-1,
pubmed-meshheading:11289809-HLA-A2 Antigen,
pubmed-meshheading:11289809-Humans,
pubmed-meshheading:11289809-Morpholines,
pubmed-meshheading:11289809-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11289809-Protein Transport,
pubmed-meshheading:11289809-Recombinant Fusion Proteins,
pubmed-meshheading:11289809-Tumor Cells, Cultured,
pubmed-meshheading:11289809-nef Gene Products, Human Immunodeficiency Virus,
pubmed-meshheading:11289809-src Homology Domains
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pubmed:year |
2001
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pubmed:articleTitle |
HIV-1 Nef blocks transport of MHC class I molecules to the cell surface via a PI 3-kinase-dependent pathway.
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pubmed:affiliation |
Departments of Medicine and Microbiology and Immunology, The University of Michigan, Ann Arbor, Michigan 48109, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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