Linked Life Data

11289064

Source:http://linkedlifedata.com/resource/pubmed/id/11289064

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 3
pubmed:dateCreated
2001-4-5
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AA858662, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF033312, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF043736, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF207904, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF207905, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AW517476, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AW518256, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D17446, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D83037, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D87660, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/P29358, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/P29361, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/P31947, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U63643
pubmed:abstractText
It was suggested that the unlimited proliferative capacity of the Echinococcus multilocularis metacestode may be related to overproduction of the 14-3-3 protein. As is known, the proliferative capacities of E. granulosus and E. multilocularis metacestodes are very different. By comparing the expression levels of the 14-3-3 gene between in vitro-obtained E. granulosus and E. multilocularis metacestodes, we were able to provide experimental evidence of the potential relation between 14-3-3 over-expression and tumour-like growth in E. multilocularis metacestodes. RT-PCR and Northern blot experiments indicated that 14-3-3 expression level is about 4-fold higher in the E. multilocularis metacestode. This differential expression was confirmed both by immunoblotting and immunocytochemistry experiments, which allowed detection of the protein in the cyst wall from E. multilocularis but not in the cyst wall from E. granulosus. The alignment of the Echinococcus 14-3-3 cDNA sequence with known 14-3-3 isoforms from other organisms, grouped the parasite sequence into the tumour growth-related isoforms. The known relation between over-expression of some 14-3-3 isoforms and tumour-related processes, together with the present results, suggest that the Echinococcus 14-3-3 protein could be one of the molecules responsible for the differences between E. granulosus and E. multilocularis metacestode growth behaviour.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0031-1820
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Comparative analysis of the 14-3-3 gene and its expression in Echinococcus granulosus and Echinococcus multilocularis metacestodes.
pubmed:affiliation
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. siles@ipa.unibe.ch
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't