Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-4-5
pubmed:databankReference
pubmed:abstractText
Swarming motility is a multicellular phenomenon comprising population migration across surfaces by specially differentiated cells. In Serratia marcescens, a network exists in which the flhDC flagellar regulatory master operon, temperature, nutrient status, and quorum sensing all contribute to the regulation of swarming motility. In this study, the rsmA (repressor of secondary metabolites) gene (hereafter rsmA(Sm)) was cloned from S. marcescens. The presence of multicopy, plasmid-encoded rsmA(Sm) expressed from its native promoter in S. marcescens inhibits swarming. Synthesis of N-acylhomoserine lactones, presumably by the product of smaI (a luxI homolog isolated from S. marcescens), was also inhibited. Knockout of rsmA(Sm) on the S. marcescens chromosome shortens the time before swarming motility begins after inoculation to an agar surface. A single copy of the chromosomal PrsmA(Sm)::luxAB reporter of rsmA(Sm) transcription was constructed. Using this reporter, the roles of the flhDC flagellar regulatory master operon, temperature and autoregulation in the control of rsmA(Sm) expression were determined. Our findings indicate that RsmA(Sm) is a component of the complex regulatory network that controls swarming.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1021-7770
pubmed:author
pubmed:copyrightInfo
Copyright 2001 National Science Council, ROC and S. Karger AG, Basel
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
160-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11287746-Amino Acid Sequence, pubmed-meshheading:11287746-Bacterial Proteins, pubmed-meshheading:11287746-Base Sequence, pubmed-meshheading:11287746-Cloning, Molecular, pubmed-meshheading:11287746-DNA-Binding Proteins, pubmed-meshheading:11287746-Escherichia coli Proteins, pubmed-meshheading:11287746-Gene Deletion, pubmed-meshheading:11287746-Gene Expression Regulation, Bacterial, pubmed-meshheading:11287746-Locomotion, pubmed-meshheading:11287746-Molecular Sequence Data, pubmed-meshheading:11287746-RNA-Binding Proteins, pubmed-meshheading:11287746-Recombinant Proteins, pubmed-meshheading:11287746-Repressor Proteins, pubmed-meshheading:11287746-Sequence Alignment, pubmed-meshheading:11287746-Serratia marcescens, pubmed-meshheading:11287746-Temperature, pubmed-meshheading:11287746-Trans-Activators, pubmed-meshheading:11287746-Transformation, Bacterial
pubmed:articleTitle
The role of RsmA in the regulation of swarming motility in Serratia marcescens.
pubmed:affiliation
School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan University, No. 1, Chan-Der Street, Taipei 100, Taiwan, ROC.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't