Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-4-3
pubmed:abstractText
The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 recognizes a nuclear antigen in HHV-6A strain GS-infected cells but not in HHV-6B strain Z29-infected cells. Secondly, we compared the reactivity of the MAb to p41/38 to that of another p41-specific MAb (MAb to p41) on immunoblots with purified p41-glutathione S-transferase fusion protein from strains GS and Z29 and GS- and Z29-infected-cell lysates. The two MAbs were tested in an enzyme-linked immunosorbent assay against a panel of synthetic peptides covering the amino acid substitutions between the GS- and Z29-derived p41 proteins, as determined by DNA sequencing of our cloned isolates of the U27 gene. The MAb to p41/38 reacted specifically with a peptide comprising p41 residues 321 to 340 from strain GS. The critical residue in this peptide was serine 328, as the substitution S328N in the Z29 strain rendered the corresponding peptide nonreactive. The p41 S328 marker was present in three of three HHV-6A strains, while four of four sequenced p41 genes from HHV-6B strains had N328. Our findings are of value for the interpretation of previous findings of p41 expression in brains of MS patients and may allow a more detailed analysis of the role of HHV-6 variants in other disorders.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-10482553, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-10482554, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-10516780, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-10619434, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-10716249, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-10765058, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-11023456, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-1653487, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-1655663, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-1851860, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-2631796, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-2786090, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-2876520, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-3259614, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-3670292, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-7594679, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-7638210, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-7687803, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-7747482, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-8122364, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-8385923, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-8389796, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-8586532, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-886304, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-8896431, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-9396611, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-9455521, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283070-9987758
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1449-55
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11283070-Amino Acid Sequence, pubmed-meshheading:11283070-Antibodies, Monoclonal, pubmed-meshheading:11283070-Antigens, Viral, pubmed-meshheading:11283070-Base Sequence, pubmed-meshheading:11283070-Cerebrospinal Fluid, pubmed-meshheading:11283070-Child, Preschool, pubmed-meshheading:11283070-DNA-Binding Proteins, pubmed-meshheading:11283070-Epitope Mapping, pubmed-meshheading:11283070-Epitopes, pubmed-meshheading:11283070-Herpesviridae Infections, pubmed-meshheading:11283070-Herpesvirus 6, Human, pubmed-meshheading:11283070-Humans, pubmed-meshheading:11283070-Infant, pubmed-meshheading:11283070-Male, pubmed-meshheading:11283070-Middle Aged, pubmed-meshheading:11283070-Molecular Sequence Data, pubmed-meshheading:11283070-Peptides, pubmed-meshheading:11283070-Sequence Analysis, DNA, pubmed-meshheading:11283070-Viral Proteins
pubmed:year
2001
pubmed:articleTitle
Definition of a divergent epitope that allows differential detection of early protein p41 from human herpesvirus 6 variants A and B.
pubmed:affiliation
Department of Virology, Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't