Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-4-3
pubmed:databankReference
pubmed:abstractText
Campylobacter jejuni is one of the most common causes of bacterial diarrhea worldwide and is the primary bacterial cause of food-borne illness. Adherence to and invasion of epithelial cells are the most important pathogenic mechanisms of Campylobacter diarrhea. Molecular characterization of invasive and noninvasive Campylobacter isolates from children with diarrhea and symptom-free children was performed by random amplified polymorphic DNA techniques (RAPD). A distinct RAPD profile with a DNA band of 1.6 kb was observed significantly more frequently among invasive (63%) than among noninvasive (16%) Campylobacter isolates (P = 0.000005). The 1.6-kb band was named the invasion-associated marker (IAM). Using specifically designed primers, a fragment of 518 bp of the iam locus was amplified in 85% of invasive and 20% of noninvasive strains (P = 0.0000000). Molecular typing with a PCR-restriction fragment length polymorphism assay which amplified the entire iam locus showed a HindIII restriction fragment polymorphism pattern associated mainly with invasive strains. Although cluster analysis of the RAPD fingerprinting showed genetic diversity among strains, two main clusters were identified. Cluster I comprised significantly more pathogenic and invasive isolates, while cluster II grouped the majority of nonpathogenic, noninvasive isolates. These data indicate that most of the invasive Campylobacter strains could be differentiated from noninvasive isolates by RAPD analysis and PCR using specific primers that amplify a fragment of the iam locus.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-10361274, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-10511517, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-10768949, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-1368370, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-1408828, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-1768121, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-1979162, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-2160431, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-2259619, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-2302354, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-2318805, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-3770943, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-7542800, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-7707339, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-7727272, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8063393, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8063406, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8360165, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8489109, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8510556, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8519321, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8525063, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-8751896, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-9025274, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-9157133, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-9163918, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-9220003, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-9227862, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-9333178, http://linkedlifedata.com/resource/pubmed/commentcorrection/11283056-9817874
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1353-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Molecular characterization of invasive and noninvasive Campylobacter jejuni and Campylobacter coli isolates.
pubmed:affiliation
Department of Infectious Diseases, National Institute of Nutrition, Mexico City, Mexico.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't