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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-4-3
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pubmed:abstractText |
The growth of blood and lymphatic vasculature is mediated in part by secreted polypeptides of the vascular endothelial growth factor (VEGF) family. The prototype VEGF binds VEGF receptor (VEGFR)-1 and VEGFR-2 and is angiogenic, whereas VEGF-C, which binds to VEGFR-2 and VEGFR-3, is either angiogenic or lymphangiogenic in different assays. We used an adenoviral gene transfer approach to compare the effects of these growth factors in adult mice. Recombinant adenoviruses encoding human VEGF-C or VEGF were injected subcutaneously into C57Bl6 mice or into the ears of nude mice. Immunohistochemical analysis showed that VEGF-C upregulated VEGFR-2 and VEGFR-3 expression and VEGF upregulated VEGFR-2 expression at 4 days after injection. After 2 weeks, histochemical and immunohistochemical analysis, including staining for the lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), the vascular endothelial marker platelet-endothelial cell adhesion molecule-1 (PECAM-1), and the proliferating cell nuclear antigen (PCNA) revealed that VEGF-C induced mainly lymphangiogenesis in contrast to VEGF, which induced only angiogenesis. These results have significant implications in the planning of gene therapy using these growth factors.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD31,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Proliferating Cell Nuclear Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vascular Endothelial...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor C,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/lymphatic vessel endothelial HA...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1524-4571
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
30
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11282897-Adenoviridae,
pubmed-meshheading:11282897-Animals,
pubmed-meshheading:11282897-Antigens, CD31,
pubmed-meshheading:11282897-Cell Division,
pubmed-meshheading:11282897-Cell Line,
pubmed-meshheading:11282897-Endothelial Growth Factors,
pubmed-meshheading:11282897-Endothelium, Lymphatic,
pubmed-meshheading:11282897-Endothelium, Vascular,
pubmed-meshheading:11282897-Gene Expression,
pubmed-meshheading:11282897-Genetic Vectors,
pubmed-meshheading:11282897-Glycoproteins,
pubmed-meshheading:11282897-Humans,
pubmed-meshheading:11282897-Immunohistochemistry,
pubmed-meshheading:11282897-Lymphokines,
pubmed-meshheading:11282897-Mice,
pubmed-meshheading:11282897-Mice, Inbred C57BL,
pubmed-meshheading:11282897-Mice, Nude,
pubmed-meshheading:11282897-Neovascularization, Physiologic,
pubmed-meshheading:11282897-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:11282897-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:11282897-Receptors, Growth Factor,
pubmed-meshheading:11282897-Receptors, Vascular Endothelial Growth Factor,
pubmed-meshheading:11282897-Recombinant Fusion Proteins,
pubmed-meshheading:11282897-Skin,
pubmed-meshheading:11282897-Vascular Endothelial Growth Factor A,
pubmed-meshheading:11282897-Vascular Endothelial Growth Factor C,
pubmed-meshheading:11282897-Vascular Endothelial Growth Factor Receptor-3,
pubmed-meshheading:11282897-Vascular Endothelial Growth Factors,
pubmed-meshheading:11282897-Vesicular Transport Proteins,
pubmed-meshheading:11282897-beta-Galactosidase
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pubmed:year |
2001
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pubmed:articleTitle |
Adenoviral expression of vascular endothelial growth factor-C induces lymphangiogenesis in the skin.
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pubmed:affiliation |
Molecular/Cancer Biology Laboratory and Ludvig Institute for Cancer Research, Haartman Institute, University of Helsinki, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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