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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-3-30
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pubmed:abstractText |
The expression of CD40L was investigated in HD involved lymph nodes by flow cytometry (FCM) and reverse transcriptase polymerase chain reaction (RT-PCR). Also an investigation of the role of CD40L in upregulation of the anti-apoptotic gene BclxL in a Hodgkin's disease (HD) derived cell line was undertaken. HD patients (n = 18) had significantly higher numbers of activated CD4+ and CD8+ T cells in the tumor microenvironment as compared to controls (n = 8). HD patients also demonstrated higher numbers of CD4+, CD8+ and CD19+ lymphocytes co-expressing CD40L as compared to controls. The CD40L signal was consistently and significantly upregulated in HD patients (n = 5) as compared to controls (n = 3) at the mRNA level. RT-PCR and FCM analysis revealed that soluble CD40L upregulated BclxL levels in the Fas-sensitive HD cell line HDLM2. We conclude that CD40L can act as an important anti-apoptotic molecule by upregulating BclxL expression in Reed-Sternberg cells of HD and may be partly responsible for their survival 'in-vivo'.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1084-9785
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11279801-Apoptosis,
pubmed-meshheading:11279801-CD40 Ligand,
pubmed-meshheading:11279801-Flow Cytometry,
pubmed-meshheading:11279801-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11279801-Hodgkin Disease,
pubmed-meshheading:11279801-Humans,
pubmed-meshheading:11279801-Lymph Nodes,
pubmed-meshheading:11279801-Lymphocyte Activation,
pubmed-meshheading:11279801-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:11279801-Neoplasm Proteins,
pubmed-meshheading:11279801-Polymerase Chain Reaction,
pubmed-meshheading:11279801-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11279801-Pseudolymphoma,
pubmed-meshheading:11279801-Recombinant Proteins,
pubmed-meshheading:11279801-Reed-Sternberg Cells,
pubmed-meshheading:11279801-T-Lymphocyte Subsets,
pubmed-meshheading:11279801-Tumor Cells, Cultured,
pubmed-meshheading:11279801-bcl-X Protein
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pubmed:year |
2001
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pubmed:articleTitle |
CD40 Ligand--an anti-apoptotic molecule in Hodgkin's disease.
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pubmed:affiliation |
Immunology Division, Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai 400 012, India.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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