Linked Life Data

11279599

Source:http://linkedlifedata.com/resource/pubmed/id/11279599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-3-30
pubmed:abstractText
RT-PCR-based amplification of transcripts expressed in cancer but not in normal non-neoplastic cells is increasingly used for the sensitive detection of rare disseminated or exfoliated cancer cells to improve cancer staging and early detection protocols. However, these assays are frequently hampered by false-positive test results due to low-level transcription of the marker genes in normal cells. To overcome these limitations, target transcripts have to be identified that are tightly suppressed in normal non-neoplastic tissues, whereas they should be actively transcribed in the respective cancer cells. Here, we tested RT-PCR assays for 7 neuroendocrine marker transcripts including NCAM, PGP 9.5, gastrin, gastrin receptor, synaptophysin, preprogastrin-releasing peptide (preproGRP) and GRP-receptor to detect rare exfoliated tumor cells in peripheral venous blood and sputum samples from patients with lung cancer. Among these preproGRP RT-PCR was the only assay with which illegitimate transcription in blood or sputum samples from healthy donors or patients with unrelated diseases did not interfere. However, it reproducibly detected up to 10 small-cell lung cancer cells diluted in either 10 ml blood or 5 ml sputum samples. Single blood and sputum samples were collected directly before diagnostic bronchoscopy from 175 patients suspected to have lung cancer. Twenty-six of these had small-cell lung cancer (SCLC). Thereof, 13 patients (50%) tested positive in the blood sample and 5 of 23 patients (22%) tested positive in the sputum sample. Moreover, among 92 patients with non-small-cell lung cancer (NSCLC) 25 patients (27%) had disseminated cancer cells in peripheral blood. Amplification of preproGRP transcripts from clinical samples is a sensitive and specific assay to detect disseminated or exfoliated lung cancer cells either in peripheral blood or sputum samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11279599-Carcinoma, Non-Small-Cell Lung, pubmed-meshheading:11279599-Carcinoma, Small Cell, pubmed-meshheading:11279599-Humans, pubmed-meshheading:11279599-Leukocytes, Mononuclear, pubmed-meshheading:11279599-Lung Neoplasms, pubmed-meshheading:11279599-Neoplasm Staging, pubmed-meshheading:11279599-Neoplastic Cells, Circulating, pubmed-meshheading:11279599-Peptides, pubmed-meshheading:11279599-Protein Precursors, pubmed-meshheading:11279599-RNA, Messenger, pubmed-meshheading:11279599-Reproducibility of Results, pubmed-meshheading:11279599-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11279599-Sensitivity and Specificity, pubmed-meshheading:11279599-Sputum, pubmed-meshheading:11279599-Tumor Cells, Cultured, pubmed-meshheading:11279599-Tumor Markers, Biological
pubmed:year
2001
pubmed:articleTitle
Sensitive detection of rare cancer cells in sputum and peripheral blood samples of patients with lung cancer by preproGRP-specific RT-PCR.
pubmed:affiliation
Division of Molecular Diagnostics and Therapy, Department of Surgery, University of Heidelberg, Heidelberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't