Linked Life Data

11279037

Source:http://linkedlifedata.com/resource/pubmed/id/11279037

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2001-5-23
pubmed:databankReference
pubmed:abstractText
The general transcription factor IID consists of the TATA-binding protein (TBP) and multiple TBP-associated factors (TAFs). Here we report the isolation of two related TAF genes from the fission yeast Schizosaccharomyces pombe as multicopy suppressors of a temperature-sensitive mutation in the ubiquitin-conjugating enzyme gene ubcP4(+). The ubcP4(ts) mutation causes cell cycle arrest in mitosis, probably due to defects in ubiquitination mediated by the anaphase-promoting complex/cyclosome. One multicopy suppressor is the previously reported gene taf72(+), whereas the other is a previously unidentified gene named taf73(+). We show that the taf73(+) gene, like taf72(+), is essential for cell viability. The taf72(+) and taf73(+) genes encode proteins homologous to WD repeat-containing TAFs such as human TAF100, Drosophila TAF80/85, and Saccharomyces cerevisiae TAF90. We demonstrate that TAF72 and TAF73 proteins are present in the same complex with TBP and other TAFs and that TAF72, but not TAF73, is associated with the putative histone acetylase Gcn5. We also show that overexpression of TAF72 or TAF73 suppresses the cell cycle arrest in mitosis caused by a mutation in the anaphase-promoting complex/cyclosome subunit gene cut9(+). These results suggest that TAF72 and TAF73 may regulate the expression of genes involved in ubiquitin-dependent proteolysis during mitosis. Our study thus provides evidence for a possible role of WD repeat-containing TAFs in the expression of genes involved in progression through the M phase of the cell cycle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17117-24
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11279037-Amino Acid Sequence, pubmed-meshheading:11279037-Anaphase, pubmed-meshheading:11279037-Animals, pubmed-meshheading:11279037-Carrier Proteins, pubmed-meshheading:11279037-Drosophila, pubmed-meshheading:11279037-Fungal Proteins, pubmed-meshheading:11279037-Histone Deacetylases, pubmed-meshheading:11279037-Molecular Sequence Data, pubmed-meshheading:11279037-Plasmids, pubmed-meshheading:11279037-Repetitive Sequences, Amino Acid, pubmed-meshheading:11279037-Repressor Proteins, pubmed-meshheading:11279037-Restriction Mapping, pubmed-meshheading:11279037-Saccharomyces cerevisiae, pubmed-meshheading:11279037-Schizosaccharomyces, pubmed-meshheading:11279037-Schizosaccharomyces pombe Proteins, pubmed-meshheading:11279037-Sequence Alignment, pubmed-meshheading:11279037-Sequence Homology, Amino Acid, pubmed-meshheading:11279037-TATA-Binding Protein Associated Factors, pubmed-meshheading:11279037-Transcription Factor TFIID
pubmed:year
2001
pubmed:articleTitle
Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex.
pubmed:affiliation
Divisions of Molecular Genetics and Mutagenesis, Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. hmitsuza@lab.nig.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't