Linked Life Data

11278915

Source:http://linkedlifedata.com/resource/pubmed/id/11278915

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2001-4-17
pubmed:abstractText
Mevalonate kinase serine/threonine residues have been implicated in substrate binding and inherited metabolic disease. Alignment of >20 mevalonate kinase sequences indicates that Ser-145, Ser-146, Ser-201, and Thr-243 are the only invariant residues with alcohol side chains. These residues have been individually mutated to alanine. Structural integrity of the mutants has been demonstrated by binding studies using fluorescent and spin-labeled ATP analogs. Kinetic characterization of the mutants indicates only modest changes in K(m)((ATP)). K(m) for mevalonate increases by approximately 20-fold for S146A, approximately 40-fold for T243A, and 100-fold for S201A. V(max) changes for S145A, S201A, and T243A are < or =3-fold. Thus, the 65-fold activity decrease associated with the inherited human T243I mutation seems attributable to the nonconservative substitution rather than any critical catalytic function. V(max) for S146A is diminished by 4000-fold. In terms of V/K(MVA), this substitution produces a 10(5)-fold effect, suggesting an active site location and catalytic role for Ser-146. The large k(cat) effect suggests that Ser-146 productively orients ATP during catalysis. K(D(Mg-ATP)) increases by almost 40-fold for S146A, indicating a specific role for Ser-146 in liganding Mg(2+)-ATP. Instead of mapping within a proposed C-terminal ATP binding motif, Ser-146 is situated in a centrally located motif, which characterizes the galactokinase/homoserine kinase/ mevalonate kinase/phosphomevalonate kinase protein family. These observations represent the first functional demonstration that this region is part of the active site in these related phosphotransferases.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12573-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11278915-Adenosine Triphosphate, pubmed-meshheading:11278915-Amino Acid Sequence, pubmed-meshheading:11278915-Amino Acid Substitution, pubmed-meshheading:11278915-Animals, pubmed-meshheading:11278915-Arabidopsis, pubmed-meshheading:11278915-Bacteria, pubmed-meshheading:11278915-Binding Sites, pubmed-meshheading:11278915-Fluorescent Dyes, pubmed-meshheading:11278915-Humans, pubmed-meshheading:11278915-Molecular Sequence Data, pubmed-meshheading:11278915-Mutagenesis, Site-Directed, pubmed-meshheading:11278915-Phosphotransferases, pubmed-meshheading:11278915-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:11278915-Recombinant Proteins, pubmed-meshheading:11278915-Sequence Alignment, pubmed-meshheading:11278915-Sequence Homology, Amino Acid, pubmed-meshheading:11278915-Serine, pubmed-meshheading:11278915-Spectrometry, Fluorescence, pubmed-meshheading:11278915-Threonine
pubmed:year
2001
pubmed:articleTitle
Investigation of invariant serine/threonine residues in mevalonate kinase. Tests of the functional significance of a proposed substrate binding motif and a site implicated in human inherited disease.
pubmed:affiliation
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.