Linked Life Data

11278542

Source:http://linkedlifedata.com/resource/pubmed/id/11278542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2001-6-11
pubmed:abstractText
In this study we demonstrate that in corneal epithelium there is cell-cell contact-regulated expression of a 145-kDa glycoprotein (GP) bearing the glycan determinant Lewis(x) (Le(x)) (Galbeta(1,4)[Fucalpha(1,3)]GlcNAc). This glycoprotein (Le(x)-GP) was expressed in confluent/contact-inhibited cultures but not in sparse cultures of corneal epithelium. In contrast, a 135-kDa glycoprotein bearing precursor, unfucosylated, lactosamine-containing glycans (Galbeta1-4GlcNAcbeta1-R) was expressed in sparse cultures. Immunofluorescence staining and confocal microscopy of confluent cultures revealed that in corneal epithelium, Le(x) antigen is located in high density at sites of cell-cell adhesion. In in vitro cell-cell adhesion assays, anti-Le(x), but not anti-sialyl-Le(x) monoclonal antibodies, inhibited the formation of corneal epithelial cell-cell adhesion. Also, when added to confluent cultures, antibodies to Le(x) disrupted the monolayer and caused tightly packed polygonal cells to round up. Analysis of the expression of Fut genes that encode alpha-1,3-fucosyltransferases, the enzymes that generate the Le(x) determinant, revealed that confluent/contact-inhibited cultures of rabbit corneal epithelium contain markedly elevated levels of Fut4 and Fut3/5/6 gene transcripts compared with sparse cultures. These data suggest that the Fut4 and Fut3/5/6 genes are targets of cell-cell contact-regulated signals and that Fut gene products direct cell-cell contact-associated expression of Le(x) on the Le(x)-GP in corneal epithelium. Immunohistochemical analysis revealed that the expression of Le(x) antigen in the epithelium of adult and developing corneas is related to the stage of differentiation of the cells. Although early differentiated cells robustly expressed Le(x), relatively undifferentiated cells did not, and the expression level was relatively low in terminally differentiated cells. Overall, these data provide evidence that a Le(x)-bearing glycoprotein plays a role through the Le(x) determinant in corneal epithelial cell-cell adhesion, and these data suggest that Le(x)-mediated cell-cell interactions contribute to mechanisms that mediate corneal epithelial cell differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21714-23
pubmed:dateRevised
2008-8-15
pubmed:meshHeading
pubmed-meshheading:11278542-Amino Acid Sequence, pubmed-meshheading:11278542-Animals, pubmed-meshheading:11278542-Antibodies, Monoclonal, pubmed-meshheading:11278542-CHO Cells, pubmed-meshheading:11278542-Carbohydrate Sequence, pubmed-meshheading:11278542-Cattle, pubmed-meshheading:11278542-Cell Adhesion, pubmed-meshheading:11278542-Cell Differentiation, pubmed-meshheading:11278542-Cells, Cultured, pubmed-meshheading:11278542-Cricetinae, pubmed-meshheading:11278542-Epithelium, Corneal, pubmed-meshheading:11278542-Fucosyltransferases, pubmed-meshheading:11278542-Humans, pubmed-meshheading:11278542-Molecular Sequence Data, pubmed-meshheading:11278542-Rabbits, pubmed-meshheading:11278542-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11278542-Sequence Alignment, pubmed-meshheading:11278542-Sequence Homology, Amino Acid, pubmed-meshheading:11278542-Trisaccharides
pubmed:year
2001
pubmed:articleTitle
Role of the Lewis(x) glycan determinant in corneal epithelial cell adhesion and differentiation.
pubmed:affiliation
New England Eye Center, Department of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't