Linked Life Data

11278372

Source:http://linkedlifedata.com/resource/pubmed/id/11278372

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2001-4-24
pubmed:abstractText
Regulation of the stability of p53 is key to its tumor-suppressing activities. mdm2 directly binds to the amino-terminal region of p53 and targets it for degradation through the ubiquitin-proteasome pathway. The coactivator protein TAF(II)31 binds to p53 at the amino-terminal region that is also required for interaction with mdm2. In this report, we demonstrate that expression of TAF(II)31 inhibits mdm2-mediated ubiquitination of p53 and increases p53 levels. TAF(II)31-mediated p53 stabilization results in activation of p53-mediated transcriptional activity and leads to p53-dependent growth arrest in fibroblasts. UV-induced stabilization of p53 coincides with an increase in p53-associated TAF(II)31 and a corresponding decrease in mdm2-p53 interaction. Non-p53 binding mutant of TAF(II)31 fails to stabilize p53. Our results suggest that direct interaction of TAF(II)31 and p53 not only mediates p53 transcriptional activation but also protects p53 from mdm2-mediated degradation, thereby resulting in activation of p53 functions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13852-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11278372-3T3 Cells, pubmed-meshheading:11278372-Animals, pubmed-meshheading:11278372-Blotting, Northern, pubmed-meshheading:11278372-Blotting, Western, pubmed-meshheading:11278372-Cell Cycle, pubmed-meshheading:11278372-Cell Division, pubmed-meshheading:11278372-Fibroblasts, pubmed-meshheading:11278372-Flow Cytometry, pubmed-meshheading:11278372-Gene Expression Regulation, pubmed-meshheading:11278372-Genes, Reporter, pubmed-meshheading:11278372-Genes, p53, pubmed-meshheading:11278372-Genetic Vectors, pubmed-meshheading:11278372-Mice, pubmed-meshheading:11278372-Mice, Inbred BALB C, pubmed-meshheading:11278372-Mutation, pubmed-meshheading:11278372-Nuclear Proteins, pubmed-meshheading:11278372-Plasmids, pubmed-meshheading:11278372-Precipitin Tests, pubmed-meshheading:11278372-Protein Binding, pubmed-meshheading:11278372-Proto-Oncogene Proteins, pubmed-meshheading:11278372-Proto-Oncogene Proteins c-mdm2, pubmed-meshheading:11278372-Suppression, Genetic, pubmed-meshheading:11278372-TATA-Binding Protein Associated Factors, pubmed-meshheading:11278372-Trans-Activators, pubmed-meshheading:11278372-Transcription, Genetic, pubmed-meshheading:11278372-Transcription Factor TFIID, pubmed-meshheading:11278372-Transcriptional Activation, pubmed-meshheading:11278372-Transfection, pubmed-meshheading:11278372-Tumor Suppressor Protein p53, pubmed-meshheading:11278372-Ubiquitins, pubmed-meshheading:11278372-Ultraviolet Rays
pubmed:year
2001
pubmed:articleTitle
Stabilization and activation of p53 by the coactivator protein TAFII31.
pubmed:affiliation
Derald H. Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New York 10029, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't