Linked Life Data

11277982

Source:http://linkedlifedata.com/resource/pubmed/id/11277982

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-3-30
pubmed:abstractText
Previous studies have demonstrated that astrocyte cultures express neuropeptide Y (NPY) in a regulated manner, namely, phorbol ester leads to an increase in proNPY-mRNA and NPY production. In this respect, the behavior of astrocytes derived from the human fetal or rat neonatal brain is similar (Regul. Pept. 75 (1998) 293). Since astrocytes can be exposed to high levels of IL-1beta, we addressed the question: Does IL-1beta regulate NPY expression by the astrocytes? Primary astrocytes derived from the human fetal or rat neonatal cortex were cultured in serum-free medium. IL-1beta, but not IL-6 or TNF-alpha, led to an increase in NPY production dose-dependently. IL-1beta action manifested in the human but not in the rat astrocytes and it was completely abolished by IL-1 receptor antagonist. The responsiveness to IL-1beta did not diminish upon sub-culture of the astrocytes (five passages). In addition, IL-1beta led to an increase in the abundance of proNPY-mRNA, which was preceded by a rapid and transient increase in cFos-mRNA and a rapid and sustained increase in JunB-mRNA. In contrast to cFos/JunB, IL-1beta did not alter the abundance of cJun-mRNA. In summary, we demonstrate that IL-1beta induction of NPY expression in astrocytes is species- and cytokine-specific and that IL-1 receptor is involved. Moreover, induction of NPY expression is preceded by a rapid increase in the expression of two transcription factors (cFos, JunB) that have been previously (Oncogene 9 (1994) 2369; J. Neurochem. 70 (1998) 1887) implicated in transcriptional regulation of the human NPY gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
896
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11277982-Animals, pubmed-meshheading:11277982-Astrocytes, pubmed-meshheading:11277982-Cells, Cultured, pubmed-meshheading:11277982-Female, pubmed-meshheading:11277982-Fetus, pubmed-meshheading:11277982-Gene Expression, pubmed-meshheading:11277982-Humans, pubmed-meshheading:11277982-Immunohistochemistry, pubmed-meshheading:11277982-Interleukin-1, pubmed-meshheading:11277982-Neuropeptide Y, pubmed-meshheading:11277982-Pregnancy, pubmed-meshheading:11277982-Protein Precursors, pubmed-meshheading:11277982-Proto-Oncogene Proteins c-fos, pubmed-meshheading:11277982-Proto-Oncogene Proteins c-jun, pubmed-meshheading:11277982-RNA, Messenger, pubmed-meshheading:11277982-Rats, pubmed-meshheading:11277982-Rats, Sprague-Dawley, pubmed-meshheading:11277982-Receptors, Interleukin-1, pubmed-meshheading:11277982-Species Specificity, pubmed-meshheading:11277982-Transcription, Genetic, pubmed-meshheading:11277982-Transcription Factor AP-1
pubmed:year
2001
pubmed:articleTitle
Interleukin-1beta induces expression of neuropeptide Y in primary astrocyte cultures in a cytokine-specific manner: induction in human but not rat astrocytes.
pubmed:affiliation
Department of Obstetrics and Gynecology, The University of Texas Southwestern Medical Center at Dallas, 5373 Harry Hines Boulevard, Dallas, TX 75390-9032, USA. ayalla.barnea@utsouthwestern.edu
pubmed:publicationType
Journal Article, Comparative Study