| pubmed-article:11276369 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0005773 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0162371 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0301944 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0332152 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0010405 | lld:lifeskim |
| pubmed-article:11276369 | lifeskim:mentions | umls-concept:C0444706 | lld:lifeskim |
| pubmed-article:11276369 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11276369 | pubmed:dateCreated | 2001-3-29 | lld:pubmed |
| pubmed-article:11276369 | pubmed:abstractText | Cytokine-mediated expansion has been proposed and successfully used to facilitate engraftment post transplantation. This study examined whether cryopreservation following expansion has a detrimental effect on the ability of cells to engraft, using the NOD-SCID mouse model. Cord blood (CB) CD34(+) cells were incubated for 7 days with stem cell factor (SCF), flt-3 ligand (FL), and megakaryocyte growth and development factor (MGDF). Expanded CD34(+) cells were transplanted into NOD-SCID mice either fresh or following cryopreservation and thawing. After thawing, recovery of nucleated cells was 94%, of CD34 cells was 63%, and of day-14 progenitors was 17%. The loss of day-14 progenitor cells among the thawed expanded cells did not influence the kinetics of human engraftment in the mouse. Bone marrow (BM) of mice transplanted with thawed expanded CD34(+) cells (14 +/- 3.9%) showed significantly higher levels of human engraftment than mice transplanted with fresh expanded CD34(+) cells (1.5 +/- 0.5%, p = 0.0064). Thawed expanded CD34(+) cells had significantly higher SCID Engrafting Potential (SEP) than freshly expanded CD34(+) cells (p < 0.001). Results suggest that prior cryopreservation does not prevent expanded cells engrafting in NOD-SCID mice. | lld:pubmed |
| pubmed-article:11276369 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11276369 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11276369 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11276369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11276369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11276369 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11276369 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11276369 | pubmed:issn | 1525-8165 | lld:pubmed |
| pubmed-article:11276369 | pubmed:author | pubmed-author:RiceA MAM | lld:pubmed |
| pubmed-article:11276369 | pubmed:author | pubmed-author:CaseJJ | lld:pubmed |
| pubmed-article:11276369 | pubmed:author | pubmed-author:CollinsC JCJ | lld:pubmed |
| pubmed-article:11276369 | pubmed:author | pubmed-author:WoodJ AJA | lld:pubmed |
| pubmed-article:11276369 | pubmed:author | pubmed-author:VowelsM RMR | lld:pubmed |
| pubmed-article:11276369 | pubmed:author | pubmed-author:MilrossC GCG | lld:pubmed |
| pubmed-article:11276369 | pubmed:author | pubmed-author:NordonR ERE | lld:pubmed |
| pubmed-article:11276369 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11276369 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:11276369 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11276369 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11276369 | pubmed:pagination | 157-65 | lld:pubmed |
| pubmed-article:11276369 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
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| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
| pubmed-article:11276369 | pubmed:meshHeading | pubmed-meshheading:11276369... | lld:pubmed |
| pubmed-article:11276369 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11276369 | pubmed:articleTitle | Prior cryopreservation of ex vivo-expanded cord blood cells is not detrimental to engraftment as measured in the NOD-SCID mouse model. | lld:pubmed |
| pubmed-article:11276369 | pubmed:affiliation | Children's Cancer Institute Australia for Medical Research, Sydney Children's Hospital, Randwick, NSW, Australia. A.Rice@unsw.edu.au | lld:pubmed |
| pubmed-article:11276369 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11276369 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:11276369 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11276369 | lld:pubmed |
