Linked Life Data

11275985

Source:http://linkedlifedata.com/resource/pubmed/id/11275985

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-3-29
pubmed:abstractText
The Epstein-Barr virus (EBV) is tightly linked to the induction of undifferentiated nasopharyngeal carcinoma (NPC), a tumour endemic in certain areas of southeast Asia. The LMP1 gene encoded by EBV is a classical oncogene due to its ability to transform rodent fibroblasts. LMP1 is absolutely essential for transformation of B cells by the virus and is one of the few EBV genes found to be expressed in NPC. It was originally shown that the LMP1 gene from NPC harbours a deletion of 30 bp in the 3' part of the gene. However, this deletion is also present in the virus spread in healthy people of the areas endemic for NPC and also in other EBV-positive tumours as well as in healthy carriers. We isolated and sequenced the LMP1 gene obtained from tissue of 7 Russian patients with NPC and 1 German patient with an NPC-like tumour of the parotid gland (PG) and compared them with the LMP1 gene isolated from peripheral blood lymphocytes (PBLs) of 6 Russian and 4 German healthy EBV-positive carriers. Neither the Russian NPC cases nor the German NPC-like tumour harboured an LMP1 gene with the 30-bp deletion, while 1 Russian and 2 German carriers contained the LMP1 gene with the 30-bp deletion. In addition, the LMP1 gene isolated from PBLs of the German patient was virtually identical to the gene isolated from the primary tumour. Functional analysis showed no correlation between the presence or absence of the 30-bp deletion and the level of induction of the transcription factors NFkappaB and jun/AP-1 caused by LMP1. These data indicate that the 30-bp deletion is not a factor predisposing for NPC. Comparison of the DNA sequences revealed that the LMP1 genes present in the NPCs most likely represent the "strain" persisting in the general population.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
815-21
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11275985-Adolescent, pubmed-meshheading:11275985-Adult, pubmed-meshheading:11275985-Alkaline Phosphatase, pubmed-meshheading:11275985-Base Sequence, pubmed-meshheading:11275985-Blotting, Western, pubmed-meshheading:11275985-DNA Primers, pubmed-meshheading:11275985-Female, pubmed-meshheading:11275985-Gene Deletion, pubmed-meshheading:11275985-Genetic Variation, pubmed-meshheading:11275985-Germany, pubmed-meshheading:11275985-Humans, pubmed-meshheading:11275985-Luciferases, pubmed-meshheading:11275985-Male, pubmed-meshheading:11275985-Middle Aged, pubmed-meshheading:11275985-Molecular Sequence Data, pubmed-meshheading:11275985-NF-kappa B, pubmed-meshheading:11275985-Nasopharyngeal Neoplasms, pubmed-meshheading:11275985-Parotid Neoplasms, pubmed-meshheading:11275985-Polymerase Chain Reaction, pubmed-meshheading:11275985-Russia, pubmed-meshheading:11275985-Sequence Homology, Nucleic Acid, pubmed-meshheading:11275985-Tumor Cells, Cultured, pubmed-meshheading:11275985-Viral Matrix Proteins
pubmed:year
2001
pubmed:articleTitle
The LMP1 gene isolated from Russian nasopharyngeal carcinoma has no 30-bp deletion.
pubmed:affiliation
Institut für Medizinische Mikrobiologie und Hygiene, Abteilung Virologie, Gebäude 47, Universitätskliniken, Homburg/Saar, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't