Linked Life Data

11272108

Source:http://linkedlifedata.com/resource/pubmed/id/11272108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-2-22
pubmed:abstractText
We have performed experiments designed to investigate the mechanism for the enhancement of radiation transformation in vitro by 12-O-tetradecanoylphorbol-13-acetate (TPA). Two types of experiments, involving C3H 10T1/2 cells and 100 rad X-ray exposures with subsequent TPA treatment, are reported here. In one set of experiments, cultures were initially seeded at differing initial cell densities prior to irradiation. In the other series of experiments, cultures exposed to 100 rads and TPA contained the same initial cell densities (about 300 viable cells per dish); these cultures were allowed to reach confluence and were then reseeded at various cell densities to allow a second cycle of growth to confluence (which involved different numbers of cell divisions). We have observed that the number of transformed foci which ultimately developed per dish treated with 100 rads and TPA remains approximately constant even though the cell density (initial or reseeded) is varied over several orders of magnitude. The yield of transformants per dish which occurred following this treatment was similar to those previously observed in cultures irradiated with higher doses (400-600 rads) of X-rays alone. Our results suggest that a similar mechanism for the induction of malignant transformation may be involved for high doses of radiation alone or for a low dose of radiation followed by TPA treatment.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1039-47
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Investigation of the mechanism for enhancement of radiation transformation in vitro by 12-O-tetradecanoylphorbol-13-acetate.
pubmed:affiliation
Department of Physiology, Harvard University, School of Public Health, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.