Source:http://linkedlifedata.com/resource/pubmed/id/11270396
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2001-3-28
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pubmed:abstractText |
Flow cytometry (FC) has the ability to discriminate a variety of cell parameters including cell size and complexity, and fluorescence intensity. As yeast cells or fungal spores germinate they undergo a morphological transformation from round oval shaped cells to elongate filamentous forms. To date, monitoring these events has been performed using microscopic examination. Microscopic examination is a labor intensive process that examines a very small percentage of the total cell population. We have developed a method using FC that is rapid, simple to perform, and reproducible. The major advantages of FC include analysis of a larger number of cells, increased objectivity due to nonselective measurements of all cells in the population studied, and the computer related data analysis capability of the flow cytometer.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1369-3786
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-7
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pubmed:meshHeading | |
pubmed:year |
2001
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pubmed:articleTitle |
The use of flow cytometry as a tool for monitoring filament formation of fungi.
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pubmed:affiliation |
Clinical Microbiology and Immunology Laboratories UNC Hospitals, Chapel Hill, NC 27514, USA. rhopfer@unch.unc.edu
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pubmed:publicationType |
Journal Article,
Evaluation Studies
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