Linked Life Data

11267658

Source:http://linkedlifedata.com/resource/pubmed/id/11267658

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-3-27
pubmed:abstractText
We have previously shown that in response to treatment with HgCl(2), the adult mouse liver exhibits both transcriptional and translational regulation of the acute phase response genes. In this study we asked whether the heavy metal treatment affects the regulation of the C/EBP transcription factors which play a key role in regulation of the acute phase response gene. Our studies have shown that the AGP gene is transcriptionally activated while transcription of the CCAAT/enhancer-binding trans-activating protein (C/EBP)alpha gene is slightly down-regulated and that of the C/EBPbeta gene does not respond. Both the C/EBPalpha and C/EBPbeta mRNAs produce multiple isoforms possibly by alternative translation initiation (ATI) of multiple internal AUG initiation sites. The C/EBPbeta mRNA appears to be stabilized. Although similar regulatory processes occur in response HgCl(2) vs. LPS, our data suggest that the translational processes (ATI) are differentially affected. In addition, a major difference lies in the fact that the C/EBPbeta gene is not transcriptionally activated by HgCl(2). Our data show decreased binding activity and pool levels of the C/EBPalpha isoform (p42(C/EBPalpha)) and increased binding activity and pool levels of C/EBPbeta isoform (p35(C/EBPbeta)) in response to HgCl(2). We propose that this isoform may be involved in the regulation of AGP gene expression in response to heavy metals and that there is a significant difference between the HgCl(2)-mediated and LPS-mediated inflammatory response.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
1518
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-56
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11267658-Alternative Splicing, pubmed-meshheading:11267658-Animals, pubmed-meshheading:11267658-CCAAT-Enhancer-Binding Protein-alpha, pubmed-meshheading:11267658-CCAAT-Enhancer-Binding Protein-beta, pubmed-meshheading:11267658-COS Cells, pubmed-meshheading:11267658-Cercopithecus aethiops, pubmed-meshheading:11267658-DNA, pubmed-meshheading:11267658-Gene Expression Regulation, pubmed-meshheading:11267658-Genetic Vectors, pubmed-meshheading:11267658-Lipopolysaccharides, pubmed-meshheading:11267658-Liver, pubmed-meshheading:11267658-Male, pubmed-meshheading:11267658-Mercuric Chloride, pubmed-meshheading:11267658-Mice, pubmed-meshheading:11267658-Mice, Inbred BALB C, pubmed-meshheading:11267658-Orosomucoid, pubmed-meshheading:11267658-Promoter Regions, Genetic, pubmed-meshheading:11267658-Protein Isoforms, pubmed-meshheading:11267658-Transcription, Genetic, pubmed-meshheading:11267658-Transfection
pubmed:year
2001
pubmed:articleTitle
Effects of mercuric chloride on the regulation of expression of the acute phase response components alpha(1)-acid glycoprotein and C/EBP transcription factors.
pubmed:affiliation
Aristotle University of Thessaloniki, School of Sciences, Biology Department, 54006 Thessaloniki, Greece.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.