Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-3-27
pubmed:abstractText
The macrophage colony stimulating factor (M-CSF) and alpha(v)beta(3) integrins play critical roles in osteoclast function. This study examines M-CSF- and adhesion-induced signaling in prefusion osteoclasts (pOCs) derived from Src-deficient and wild-type mice. Src-deficient cells attach to but do not spread on vitronectin (Vn)-coated surfaces and, contrary to wild-type cells, their adhesion does not lead to tyrosine phosphorylation of molecules activated by adhesion, including PYK2, p130(Cas), paxillin, and PLC-gamma. However, in response to M-CSF, Src(-/-) pOCs spread and migrate on Vn in an alpha(v)beta(3)-dependent manner. Involvement of PLC-gamma activation is suggested by using a PLC inhibitor, U73122, which blocks both adhesion- and M-CSF-mediated cell spreading. Furthermore, in Src(-/-) pOCs M-CSF, together with filamentous actin, causes recruitment of beta(3) integrin and PLC-gamma to adhesion contacts and induces stable association of beta(3) integrin with PLC-gamma, phosphatidylinositol 3-kinase, and PYK2. Moreover, direct interaction of PYK2 and PLC-gamma can be induced by either adhesion or M-CSF, suggesting that this interaction may enable the formation of integrin-associated complexes. Furthermore, this study suggests that in pOCs PLC-gamma is a common downstream mediator for adhesion and growth factor signals. M-CSF-initiated signaling modulates the alpha(v)beta(3) integrin-mediated cytoskeletal reorganization in prefusion osteoclasts in the absence of c-Src, possibly via PLC-gamma.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10029582, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10354709, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10430888, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10446041, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10473632, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10547359, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10559917, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10660045, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10676810, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10761925, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-10806474, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-1383278, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-1385441, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-1997203, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7525598, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7540546, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7541382, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7545020, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7612963, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7657702, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7682059, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7929388, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-7962166, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-8054683, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-8388373, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-8573343, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-8573791, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-8799848, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-8954718, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9028319, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9028946, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9281348, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9330877, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9353253, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9366562, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9434625, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9443896, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9506840, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9529375, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9561851, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9682033, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9716455, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9722509, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9727056, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9733717, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9804818, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9827577, http://linkedlifedata.com/resource/pubmed/commentcorrection/11266452-9988732
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie..., http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes, http://linkedlifedata.com/resource/pubmed/chemical/Bcar1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Crk-Associated Substrate Protein, http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Estrenes, http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 2, http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes, http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor, http://linkedlifedata.com/resource/pubmed/chemical/Paxillin, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase C gamma, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ptk2b protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Pxn protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vitronectin, http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Like Protein p130, http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases, http://linkedlifedata.com/resource/pubmed/chemical/Vitronectin, http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases, http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
152
pubmed:owner
NLM
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